The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance
The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance
复制标题
DOI：
10.1016/j.devcel.2008.07.007
复制
发表时间：
2008-09-16
影响因子：
11.8
通讯作者：
Weisman, Lois S.
中科院分区：
文献类型：
--
作者：
Peng, Yutian;Weisman, Lois S.
在芽殖酵母中，液泡遗传与细胞周期紧密协调。空泡和其他几种细胞器的运动是基于肌动蛋白的，并通过酵母肌球蛋白V马达Myo2和细胞器特异性衔接子之间的相互作用介导。Myo2通过衔接蛋白Vac17与液泡结合，衔接蛋白Vac17与液泡膜蛋白Vac8结合。在这里，我们表明，酵母细胞周期蛋白依赖性激酶Cdk1磷酸化Vac17和Vac17的磷酸化平行的细胞周期依赖性运动的液泡。Vac17中Cdk1位点的取代降低了其与Myo2的相互作用，并导致液泡遗传的部分缺陷。这种缺陷在具有突变磷酸化位点的Myo2存在下增强。因此，Cdk 1似乎控制液泡运动的时间。在其他细胞器特异性肌球蛋白V衔接子中存在多个预测的Cdk1位点表明，其他细胞质细胞器的遗传可能受到类似机制的调控。
In budding yeast, vacuole inheritance is tightly coordinated with the cell cycle. The movement of vacuoles and several other organelles is actin-based and is mediated by interaction between the yeast myosin V motor Myo2 and organelle-specific adaptors. Myo2 binds to vacuoles via the adaptor protein Vac17, which binds to the vacuole membrane protein Vac8. Here we show that the yeast cyclin-dependent kinase Cdk1 phosphorylates Vac17 and that phosphorylation of Vac17 parallels cell cycle-dependent movement of the vacuole. Substitution of the Cdk1 sites in Vac17 decreases its interaction with Myo2 and causes a partial defect in vacuole inheritance. This defect is enhanced in the presence of Myo2 with mutated phosphorylation sites. Thus, Cdk1 appears to control the timing of vacuole movement. The presence of multiple predicted Cdk1 sites in other organelle-specific myosin V adaptors suggests that the inheritance of other cytoplasmic organelles may be regulated by a similar mechanism.