Polyphenol-enriched Vaccinium uliginosum L. fractions reduce retinal damage induced by blue light in A2E-laden ARPE19 cell cultures and mice
Polyphenol-enriched Vaccinium uliginosum L. fractions reduce retinal damage induced by blue light in A2E-laden ARPE19 cell cultures and mice
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DOI：
10.1016/j.nutres.2016.11.008
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发表时间：
2016-12-01
影响因子：
4.5
通讯作者：
Choung, Se-Young
中科院分区：
文献类型：
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作者：
Lee, Bom-Lee;Kang, Jung-Hwan;Choung, Se-Young
多酚对视力产生有益的影响。我们假设越橘 (V.U.) 提取物的多酚成分可以保护视网膜色素上皮 (RPE) 细胞免受蓝光引起的损伤。我们的目的是测试含有多酚成分的提取物，以确定减少 RPE 蓝光损伤的效果。我们测量了从水、乙醇和 HP20 树脂 (FH) 洗脱的级分的活性，发现 FH 级分具有最高的有益活性。我们使用色谱技术从 FH 级分中分离出各个活性化合物，发现 FH 含有类黄酮、花青素、苯丙素和环烯醚萜。载有 A2E 的 ARPE-19 细胞培养物经 V.U. 处理后暴露于蓝光。提取物级分及其各自的成分表明有所改善。 V uliginosum L 提取物组分和组成化合物显着减少 A2E 光氧化诱导的 RPE 细胞死亡并抑制细胞内 A2E 积累。此外，Balb/c雄性小鼠暴露于10000勒克斯的蓝光下1小时/天，持续两周，以诱导视网膜损伤。最后一次蓝光照射一周后，视网膜损伤评估显示外核层厚度和细胞核数量有所改善。小鼠感光细胞的组织学检查表明，富含多酚的 FH 可以抑制外核层厚度和细胞核的损失。我们的研究结果表明，V.U.提取物和洗脱组分是生物活性化合物的潜在来源，有可能成为年龄相关性黄斑变性的治疗方法。 (C) 2016 Elsevier Inc. 保留所有权利。
Polyphenols exert beneficial effects on vision. We hypothesized that polyphenol components of Vaccinium uliginosum L. (V.U.) extract protect retinal pigment epithelial (RPE) cells against blue light-induced damage. Our aim was to test extracts containing polyphenol components to ascertain effects to reduce damage against blue light in RPEs. We measured the activity in fractions eluted from water, ethanol, and HP20 resin (FH), and found that the FH fraction had the highest beneficial activity. We isolated the individual active compounds from the FH fraction using chromatographic techniques, and found that FH contained flavonoids, anthocyanins, phenyl propanoids, and iridoids. Cell cultures of A2E-laden ARPE-19 exposed to blue light after treatment with V.U. extract fractions and their individual constituents indicated improvement. V uliginosum L extract fractions and constituent compounds significantly reduced A2E photo-oxidation-induced RPE cell death and inhibited intracellular A2E accumulation. Furthermore, Balb/c male mice were exposed to blue light at 10000 lux for 1 h/d for 2 weeks to induce retinal damage. One week after the final blue light exposure, retinal damage evaluated revealed that the outer nuclear layer thickness and nuclei count were improved. Histologic examination of murine photoreceptor cells demonstrated that FH, rich in polyphenols, inhibited the loss of outer nuclear layer thickness and nuclei. Our findings suggest that V.U. extract and eluted fractions are a potential source of bioactive compounds that potentially serve a therapeutic approach for age-related macular degeneration. (C) 2016 Elsevier Inc. All rights reserved.