Effect of dynamic hydrostatic pressure on rabbit intervertebral disc cells
Effect of dynamic hydrostatic pressure on rabbit intervertebral disc cells
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DOI：
10.1016/s0736-0266(03)00027-5
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发表时间：
2003-07-01
影响因子：
2.8
通讯作者：
Buckwalter, J
中科院分区：
文献类型：
--
作者：
Kasra, M;Goel, V;Buckwalter, J
在细胞水平上，振动诱发的腰椎间盘疾病的发病机制还很不清楚。本研究的目的是建立一种方法来研究在防止或诱导细胞外间盘基质降解中建设性和破坏性静水载荷的频率和幅度的范围。采用液压试验室，对正常兔腰椎间盘细胞进行了动态静水压载荷试验。对椎间盘外环细胞单层培养和髓核细胞海藻酸盐三维培养进行了检测。观察不同加载幅度(3-D培养，0-3 Mpa；单层，0-1.7 Mpa)和频率(1-20 Hz)对间盘胶原和蛋白质代谢的影响，通过测定细胞外基质中与细胞相关的~H-Pro标记蛋白和释放H-3-Pro标记分子到培养液中。高频、高幅值静水应激可促进体外培养的外环细胞合成胶原，而低幅值、高幅值静水应力对外环细胞胶原合成影响不大。在相同的加载时间和重复次数下，两种处理都不会显著影响细胞层蛋白质的相对释放量，说明蛋白质的降解和稳定性没有受到影响。在三维核培养中，较高的振幅和频率提高了合成速率，降低了降解。在这种情况下，加载幅度对细胞响应的影响比加载频率对细胞响应的影响更大。考虑到本研究所使用的加载幅度和频率范围，短期施加较高的加载幅度和频率有利于促进蛋白质合成和减少蛋白质降解。(C)2003年骨科研究会。爱思唯尔科学有限公司出版。版权所有。
The pathogenesis of vibration-induced disorders of intervertebral disc at the cellular level is largely unknown. The objective of this study was to establish a method to investigate the ranges of constructive and destructive hydrostatic loading frequencies and amplitudes in preventing or inducing extracellular disc matrix degradation. Using a hydraulic chamber, normal rabbit intervertebral disc cells were tested under dynamic hydrostatic loading. Monolayer cultures of disc outer annulus cells and 3-dimensional (3-D) alginate cultures of disc nucleus pulposus cells were tested. Effects of different loading amplitudes (3-D culture, 0-3 MPa; monolayer, 0-1.7 MPa) and frequencies (1-20 Hz) on disc collagen and protein metabolism were investigated by measuring 3 H-proline-labeled proteins associated with the cells in the extracellular matrix and release of H-3-proline-labeled molecules into culture medium. High frequency and high amplitude hydrostatic stress stimulated collagen synthesis in cultures of outer annulus cells whereas the lower amplitude and frequency hydrostatic stress had little effect. For the same loading duration and repetition, neither treatment significantly affected the relative amount of protein released from the cell layers, indicating that protein degradation and stability were unaffected. In the 3-D nucleus culture, higher amplitude and frequency increased synthesis rate and lowered degradation. In this case, loading amplitude had a stronger influence on cell response than that of loading frequency. Considering the ranges of loading amplitude and frequency used in this study, short-term application of high loading amplitudes and frequencies was beneficial in stimulation of protein synthesis and reduction of protein degradation. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.