Visualization of Boronic Acid Containing Pharmaceuticals in Live Tumor Cells Using a Fluorescent Boronic Acid Sensor
Visualization of Boronic Acid Containing Pharmaceuticals in Live Tumor Cells Using a Fluorescent Boronic Acid Sensor
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DOI：
10.1021/acssensors.6b00522
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发表时间：
2016-12-01
期刊：
影响因子：
8.9
通讯作者：
Kirihata, Mitsunori
中科院分区：
文献类型：
--
作者：
Hattori, Yoshihide;Ishimura, Miki;Kirihata, Mitsunori
含硼酸化合物被认为是一类新型药物，其在活细胞中的分布对于新药的开发至关重要。然而，硼化合物的通用检测方法不适合活细胞成像，并且荧光标记技术在小分子应用中具有挑战性。基于硼螯合配体的荧光硼传感器 DAHMI 是为活细胞中硼基药物的新型可视化方法而开发的。在这项研究中，我们使用荧光硼酸传感器分析了活细胞中硼酸衍生物的分布。
Boronic acid containing compounds are regarded as a new class of pharmaceuticals, and the distribution in live cells is crucial for the development of novel drugs. However, generic detection methods for boron compounds are not suitable for live-cell imaging, and the fluorescence-labeling technique is challenging to apply for small molecules. Fluorescent boron sensor DAHMI which is based on a boron chelating ligand was developed for a novel visualization method of boron-based pharmaceuticals in the live cell. In this study, we analyzed the distribution of boronic acid derivatives in live cells using a fluorescent boronic acid sensor.