Resistance of surface-dried virus to common disinfection procedures
Resistance of surface-dried virus to common disinfection procedures
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DOI：
10.1016/j.jhin.2007.05.005
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发表时间：
2007-08-01
影响因子：
6.9
通讯作者：
Wout, A. B. van 't
中科院分区：
文献类型：
--
作者：
Terpstra, F. G.;van den Blink, A. E.;Wout, A. B. van 't
据信，表面干燥的病毒仍然具有传染性，因此可能对公众健康构成威胁。为了帮助解决这个问题，我们研究了0.1N NaOH和0.1%次氯酸盐在血浆或培养介质中对表面干燥的脂膜(LE)[人类免疫缺陷病毒(HIV)、牛病毒性腹泻病毒(BVDV)和伪狂犬病病毒(PRV)]和非脂膜[NLE；犬细小病毒(CPV)和甲型肝炎病毒(HAV)]病毒的灭活能力。此外，80%乙醇在表面干燥的LE病毒上进行了试验。在没有治疗的情况下，表面干燥的LE病毒至少保持一周的传染性，而NLE病毒保持一个多月的传染性。无论使用何种消毒剂，在血浆中干燥的病毒灭活能力都有所下降，这比培养上清液中的病毒更能代表病毒污染的血液。所有消毒剂的灭活在补水之前都会改善，尽管在补水和消毒后CPV的传染性实际上增加了，因此在没有补水的情况下可能高估了CPV的传染性。这是首次在表面干燥状态下对五种重要(模型)病毒进行的全面研究，这些病毒显示出传染性的持久性、对三种常用消毒剂的抗药性以及在复水后恢复敏感性。我们的结果可能对预防病毒传播的卫生措施有一定的指导意义。(C)2007年医院感染学会。爱思唯尔有限公司出版。保留所有权利。
It is believed that surface-dried viruses can remain infectious and may therefore pose a threat to public health. To help address this issue, we studied 0.1 N NaOH and 0.1% hypochlorite for their capacity to inactivate surface-dried lipid-enveloped (LE) [human immunodeficiency virus (HIV), bovine viral diarrhoea virus (BVDV) and pseudorabies virus (PRV)] and non-lipid-enveloped [NLE; canine parvovirus (CPV) and hepatitis A virus (HAV)] viruses in a background of either plasma or culture medium. In addition, 80% ethanol was tested on surface-dried LE viruses. Without treatment, surface-dried LE viruses remained infectious for at least one week and NLE viruses for more than one month. Irrespective of the disinfectant, inactivation decreased for viruses dried in plasma, which is more representative of viral contaminated blood than virus in culture medium. Inactivation by all disinfectants improved when preceded by rehydration, although the infectivity of CPV actually increased after rehydration and disinfection may thus be overestimated in the absence of rehydration. This is the first comprehensive study of five important (model) viruses in a surface-dried state showing persistence of infectivity, resistance to three commonly used disinfectants and restoration of susceptibility after rehydration. Our results may have implications for hygiene measurements in the prevention of virus transmission. (c) 2007 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.