Highly sensitive and rapid sequential cortisol detection using twin sensor QCM
Highly sensitive and rapid sequential cortisol detection using twin sensor QCM
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DOI：
10.1039/c4ay01387e
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Suzuki, Koji
中科院分区：
文献类型：
--
作者：
Ito, Takeshi;Aoki, Nobuyoshi;Suzuki, Koji
为了适应序列分析的需要，提出了一种结合流动注射分析的快速皮质醇检测系统。该检测系统由石英晶体微平衡（QCM）方法的双传感器芯片组成。一个传感器监测抗原-抗体相互作用，另一个传感器作为参考，以消除环境影响。竞争性试验进行了监测抗原-抗体相互作用使用皮质醇偶联牛血清白蛋白（rt- bsa）作为示踪剂。抗原和抗体之间的结合可以用甘氨酸-氢氧化钠溶液解耦，以适应序列分析。在6 μ L min（-1）的流速下，在10分钟内完成一个检测周期，包括再生步骤。传感器系统可以定量检测皮质醇水平，范围从5 pg mL（-1）到100 pg mL（-1）。这些结果表明，该传感器系统具有用于主动应力监测和疾病诊断的潜力。
We present a rapid cortisol detecting system with the integration of flow injection analysis in order to adapt sequential analysis. The detecting system consists of a twin sensor chip on quartz crystal micro balance (QCM) method. One sensor monitored antigen-antibody interaction, together with the other sensor as a reference to remove environmental influences. A competitive assay was performed on monitoring the antigen-antibody interaction using cortisol coupled with bovine serum albumin (Crt-BSA) as a tracer. The binding between antigen and antibody could be uncoupled with a glycine-NaOH solution to adapt sequential analysis. One detection cycle, including regeneration step was performed within 10 minutes at a flow rate of 6 mu L min(-1). The sensor system could quantitatively detect cortisol levels ranging from 5 pg mL(-1) to 100 pg mL(-1). These results indicated the sensor system has the potential to be used for active stress-monitoring and in the diagnosis of disease.