Antimicrobial Activity and Identification of Actinomycete Strains from aFolk Medicinal Soil in China
Antimicrobial Activity and Identification of Actinomycete Strains from aFolk Medicinal Soil in China
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DOI：
10.4172/2471-9315.1000105
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Li Xu;Ganjun Yuan;Q. Zeng;Xiaoyi Zhu
中科院分区：
文献类型：
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作者：
Li Xu;Ganjun Yuan;Q. Zeng;Xiaoyi Zhu
Objective: To discover novel antimicrobial medicinal resource and reveal the anti-infection reasons of a folk medicinal soil. Methods: The antimicrobial activities of sixty-one actinomycete strains isolated from a folk medicinal soil sample were evaluated using agar well diffusion and broth microdilution methods, and then seven strains with broadspectrum antimicrobial activities were identified using polyphasic taxonomy procedure. Results: Thirteen strains presented antimicrobial activities, and seven strains showed broad-spectrum antimicrobial activities with the minimum inhibitory concentrations (MICs) of 0.256-4.096 mg/ml against four pathogenic microorganisms including methicillin-susceptible Staphylococcus aureus, methicillin-resistant S. aureus (MRSA) and Candida albicans. Moreover, strain ZZ035 also showed antimicrobial activities with the MICs of 0.256-1.024 mg/ml against Escherichia coli. The identifications of these seven strains showed that all of them belonged to the genus Streptomyces. Furthermore, strain ZZ016 presented a high potential to be a new species. Conclusion: These streptomycete strains were deduced to be mainly responsible for the anti-infection effect of the folk medicinal soil, and further research on their secondary metabolites may lead to the discovery of remarkable and broad-spectrum antibiotics, especially that of anti-MRSA ones.
