Changes in glial fibrillary acidic protein (GFAP) immonureactivity reflect neuronal states
Changes in glial fibrillary acidic protein (GFAP) immonureactivity reflect neuronal states
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DOI：
10.1007/s11064-008-9745-2
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Hajos, Ferenc
中科院分区：
文献类型：
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作者：
Hajos, Ferenc
通过免疫组织化学研究了星形胶质细胞标记物胶质细胞酸性蛋白（GFAP）在不同脑区的变化，以观察其在不同功能状态下的波动。不同的神经元状态，无论是实验诱导或在生理条件下研究。为了产生实验性的改变，视觉系统被选为模型。在病变的外侧膝状体与鹅膏蕈氨酸的立体定位注射的GFAP免疫反应性的增加，可以诱导在同侧视觉皮层的第三层和第四层的膝状体皮质纤维终止。电子显微镜显示突触末梢的同步变性和突触周星形胶质细胞突起的肥大。为了研究完整动物的变化，观察了照明的影响。在外侧膝状体的背侧亚核被发现免疫阴性时，在白天和夜间阳性。在视交叉上核也观察到类似的变化。至于更广泛的影响，研究了性腺类固醇对GFAP反应脚间核（不参与激素调节机制的区域）的影响。在男性中，只有阉割可以降低持续高GFAP免疫反应性，而在女性GFAP显示大范围的性周期相关的波动。结论：GFAP免疫反应性的变化可以指示脑内特定区域内的突触事件。
The astroglial marker, glial fibrillary acidic protein (GFAP) was investigated by immunohistochemistry in various brain areas in order to see its fluctuations in various functional states. Different neuronal states were either experimentally induced or studied under physiological conditions. To produce experimental alterations the visual system was chosen as a model. Upon lesioning of the lateral geniculate body with the stereotaxic injection of ibotenic acid an increase in GFAP immunoreactivity could be induced in layers III and IV of the ipsilateral visual cortex where geniculo-cortical fibres terminate. Electron microscopy has revealed a synchronous degeneration of synaptic terminals and the hypertrophy of perisynaptic astrocyte processes. To study changes in the intact animal the effect of illumination was observed. In the lateral geniculate body the dorsal subnucleus was found immunonegative when studied at day and positive at night. Similar changes were observed in the suprachiasmatic nucleus. As to more generalized influences, the effect of gonadal steroids on the GFAP-reaction interpeduncular nucleus, an area not involved in hormonal regulatory mechanisms was studied. In males only castration could reduce constantly high GFAP immonoreactivity, whereas in females GFAP showed wide-range sexual cycle-related fluctuations. It was concluded that changes in GFAP immunoreactivity can indicate synaptic events whithin a circumscribed area of the brain.