T regulatory cells and Th1/Th2 cytokines in peripheral blood from tuberculosis patients
T regulatory cells and Th1/Th2 cytokines in peripheral blood from tuberculosis patients
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DOI：
10.1007/s10096-010-0908-0
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发表时间：
2010-06-01
影响因子：
4.5
通讯作者：
Shi, B-Y
中科院分区：
文献类型：
--
作者：
He, X-Y;Xiao, L.;Shi, B-Y
约10%的结核分枝杆菌感染者发展为活动性结核病（TB），Th 1效应细胞和Th 1细胞因子在控制结核分枝杆菌感染中起关键作用。肺结核感染。在这里，我们假设这种易感性M。结核病感染与TB患者中增加的T调节（Treg）细胞和Th 2细胞因子有关。为了验证这一点，我们招募了101名参与者（71名结核病患者，12名非结核性肺病患者和18名健康受试者），并研究了外周血中的Treg细胞和Th 1/Th 2细胞因子。与健康受试者相比，TB患者的CD 4（+）CD 25（+）T细胞和CD 4（+）CD 25（+）FoxP 3（+）T细胞显著增加，IL-5显著降低。与健康受试者相比，培养和痰涂片阳性肺结核（PTB（+））患者的CD 8（+）CD 28（-）T细胞、IFN-γ、TNF-α、IL-10和IL-4显著升高。化疗1个月后，PTB（+）患者的CD 4（+）CD 25（+）FoxP 3（+）和CD 8（+）CD 28（-）T细胞明显减少。肺外结核患者化疗1个月后，CD 4（+）、CD 4（+）CD 25（+）、CD 8（+）CD 28（+）T细胞明显升高。这些结果表明M.结核感染诱导循环中CD 4（+）CD 25（+）FoxP 3（+）和CD 8（+）CD 28（-）T细胞扩增，这可能与M.结核感染，以及效应免疫反应和抑制免疫反应之间的平衡是至关重要的控制M。肺结核感染。
About 10% of people infected with Mycobacterium tuberculosis develop active tuberculosis (TB), and Th1 effector cells and Th1 cytokines play key roles in controlling M. tuberculosis infection. Here, we hypothesise that this susceptibility to M. tuberculosis infection is linked to increased T regulatory (Treg) cells and Th2 cytokines in TB patients. To test this, we recruited 101 participants (71 TB patients, 12 non-TB pulmonary diseases and 18 healthy subjects) and investigated Treg cells and Th1/Th2 cytokines in peripheral blood. CD4(+)CD25(+) T cells and CD4(+)CD25(+)FoxP3(+) T cells significantly increased and IL-5 dramatically decreased in TB patients relative to healthy subjects. CD8(+)CD28(-) T cells, IFN-gamma, TNF-alpha, IL-10 and IL-4 significantly increased in patients with culture and sputum smear-positive pulmonary TB (PTB(+)) compared with healthy subjects. CD4(+)CD25(+)FoxP3(+) and CD8(+)CD28(-) T cells significantly decreased in PTB(+) after one month of chemotherapy. CD4(+), CD4(+)CD25(+) and CD8(+)CD28(+) T cells significantly increased in extra-pulmonary TB patients after one month of chemotherapy. These findings suggest that M. tuberculosis infection induces circulating CD4(+)CD25(+)FoxP3(+) and CD8(+)CD28(-) T cell expansion, which may be related to the progression of M. tuberculosis infection, and that the balance between effector immune responses and suppression immune responses is essential to control M. tuberculosis infection.