QUANTITATIVE-DETERMINATION OF SPERMATOZOA PENETRATION OF THE PERIVITELLINE LAYER OF THE HENS OVUM AS ASSESSED ON OVIPOSITED EGGS
QUANTITATIVE-DETERMINATION OF SPERMATOZOA PENETRATION OF THE PERIVITELLINE LAYER OF THE HENS OVUM AS ASSESSED ON OVIPOSITED EGGS
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DOI：
10.3382/ps.0741875
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发表时间：
1995-11-01
期刊：
影响因子：
4.4
通讯作者：
HOWARTH, B
中科院分区：
文献类型：
--
作者：
BRAMWELL, RK;MARKS, HL;HOWARTH, B
建立了一种在活体条件下测定鸡卵中穿透卵周层（PL）的精子数的方法。进行了两项试验来测试这种技术，并建立生育力和精子穿透力（SP）之间的相关值。首先，三个雅典加拿大随机繁殖的男性，以前测试为具有高生育力（100%），每个人都与七只母鸡。精子穿透率是从3天内产下的卵子（n = 41）中测定的，穿透位于胚盘（GD; 1.35 mm（2）面积）和非胚盘（NGD）面积上的PL的精子平均数分别为162.8和8.4。移除雄性动物后，监测SP以确定其持续时间，平均每天每只雄性动物分析4.6个卵。在此期间，GD和NGD区域的平均精子穿透率分别从167.0降至0.2和从9.2降至0。GD和NGD FL的平均SP持续时间分别为15.7和11.3 d。育性持续期为14.0d。GD PL的精子穿透率与自然交配母鸡产蛋的生育力呈正相关（r = .89，P < .0001）。在第二次试验中，三组（1，2，或3）的16只母鸡（35周龄）人工授精，每周连续4周与100，50，或25百万精子/50 μ L，分别。每周重复授精，连续12周。从三个4周周期的每个周期获得平均值，并用作重复。第1、2和3组GD PL的平均SP值分别为40.2、19.5和14.1，生育力值分别为95.8、92.4和83.3%。每个重复的平均值是从授精后每天每组24个卵中获得的。采用母鸡人工授精建立了GD PL的SP与生育力之间的显著相关性（r = 0.90，P <0.0001）。
A technique was developed to assess the number of cock spermatozoa penetrating the perivitelline layer (PL) in oviposited eggs in vivo. Two trials were conducted to test this technique and to establish correlation values between fertility and sperm penetration (SP). First, three Athens Canadian Randombred males, previously tested as having high fertility (100%), were each housed with seven hens. Sperm penetration was determined from eggs laid over a 3-d period (n = 41) with the mean number of spermatozoa penetrating the PL overlying the germinal disc (GD; 1.35 mm(2) area) and nongerminal disc (NGD) areas being 162.8 and 8.4, respectively. Following removal of the males, SP was monitored to establish its duration with an average of 4.6 eggs analyzed per male per day. Mean sperm penetration during this period declined from 167.0 to .2 and from 9.2 to 0 for the GD and NGD regions, respectively. The mean duration of SP was 15.7 and 11.3 d for the GD and NGD FL, respectively. The duration of fertility was also established to be 14.0 d. There was a positive correlation between sperm penetration of the GD PL and fertility from eggs laid by naturally mated hens (r = .89, P < .0001). In the second trial, three groups (1, 2, or 3) of 16 hens (35 wk of age) each were artificially inseminated weekly for 4 consecutive wk with either 100, 50, or 25 million sperm/50 mu L, respectively. Inseminations were repeated weekly for 12 consecutive wk. Mean values were obtained from each of three 4-wk periods and used as replicates. Mean SP values from the GD PL for Groups 1, 2, and 3 were 40.2, 19.5, and 14.1, with fertility values of 95.8, 92.4, and 83.3%, respectively. Each replicate mean was obtained from similar to 24 eggs per group per day postinsemination. A significant correlation between SP of the GD PL and fertility (r = .90, P < .0001) was established using artificial insemination of hens.