Stress Response Versus Stress Tolerance: A Transcriptome Analysis of Two Rice Lines Contrasting in Tolerance to Phosphorus Deficiency
Stress Response Versus Stress Tolerance: A Transcriptome Analysis of Two Rice Lines Contrasting in Tolerance to Phosphorus Deficiency
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DOI：
10.1007/s12284-009-9032-0
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发表时间：
2009-12-01
期刊：
影响因子：
5.5
通讯作者：
Wissuwa, Matthias
中科院分区：
文献类型：
--
作者：
Pariasca-Tanaka, Juan;Satoh, Kouji;Wissuwa, Matthias
转录谱分析已经确定了与磷（P）缺乏的适应性反应相关的基因;然而，区分应激反应和耐受性一直很困难。我们报告了两个水稻基因型（日本晴和NIL 6 -4，携带一个主要的耐缺磷QTL（Pup 1））在土壤中生长/不施磷肥的基因表达模式。我们测试了NIL 6 -4的耐受性与以下假设相关：（1）内部P再动员/再分配;（2）增强P溶解和/或获取;和（3）使P拦截最大化的根生长修饰。磷供应的基因远远超过那些不同的基因型。与内部P再动员/再分配和土壤P溶解/吸收相关的基因是应激反应的，但往往在不耐受的日本晴中更是如此。然而，与根细胞壁疏松和根毛延伸相关的基因puplatin（木葡聚糖内切转糖基化酶/水解酶和NAD（P）H依赖性氧化还原酶）在耐受性NIL 6 -4的根中显示出更高的表达。这得到了表型数据的支持，表型数据显示NIL 6 -4的根生物量和毛长更高。
Transcriptional profiling has identified genes associated with adaptive responses to phosphorus (P) deficiency; however, distinguishing stress response from tolerance has been difficult. We report gene expression patterns in two rice genotypes (Nipponbare and NIL6-4 which carries a major QTL for P deficiency tolerance (Pup1)) grown in soil with/without P fertilizer. We tested the hypotheses that tolerance of NIL6-4 is associated with (1) internal P remobilization/redistribution; (2) enhanced P solubilization and/or acquisition; and (3) root growth modifications that maximize P interception. Genes responding to P supply far exceeded those differing between genotypes. Genes associated with internal P remobilization/redistribution and soil P solubilization/uptake were stress responsive but often more so in intolerant Nipponbare. However, genes putatively associated with root cell wall loosening and root hair extension (xyloglucan endotrans-glycosylases/hydrolases and NAD(P) H-dependent oxidoreductase) showed higher expression in roots of tolerant NIL6-4. This was supported by phenotypic data showing higher root biomass and hair length in NIL6-4.