The Role of Melanocortins in Adipocyte Function
The Role of Melanocortins in Adipocyte Function
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DOI：
10.1111/j.1749-6632.1999.tb08666.x
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发表时间：
1999-10
影响因子：
5.2
通讯作者：
B. Boston
中科院分区：
文献类型：
--
作者：
B. Boston
摘要：已经证明，脂肪细胞表达高亲和力ACTH和α-MSH结合位点，并且ACTH、α-MSH和β-LPH是有效的脂解激素。然而，在对黑皮质素的脂解反应中存在相当大的物种差异。最近，在小鼠脂肪细胞和3 T3-L1小鼠胚胎成纤维细胞系中均发现了MC 2和MC 5受体-mRNA，但仅在3 T3-L1细胞分化为脂肪细胞后才发现。使用3 T3-L1细胞系原位表征MC 2和MC 5受体的药理学特性。小鼠MC 2和MC 5受体在脂肪细胞中均具有功能，尽管MC 5受体需要高剂量的α-MSH来激活环化酶。ACTH有效刺激环化酶，其EC 50值与以下假设一致：鼠MC 2受体而非MC 5受体通过垂体释放ACTH介导应激诱导的脂解。
ABSTRACT: It has been demonstrated that adipocytes express high affinity ACTH and α‐MSH binding sites, and that ACTH, α‐MSH, and β‐LPH are potent lipolytic Hormones. Considerable species variability exists in the lipolytic response to melanocortins, however. Recently, MC2 and MC5 receptor‐MRNA was found in both murine adipocytes and in the 3T3‐L1 murine embryonic fibroblast cell line, but only after the 3T3‐L1 cells had differentiated into adipocytes. The 3T3‐L1 cell line was used to characterize the pharmacological properties of both MC2 and MC5 receptors in situ. Both murine MC2 and MC5 receptors are functional in the adipocyte, although the MC5 receptor required high doses of α‐MSH to activate cylase. ACTH potently stimulates cyclase with EC50 values that are consistent with the hypothesis that the murine MC2 receptor, not the MC5 receptor, mediates stress‐induced lipolysis via release of ACTH from the pituitary.