Insertion of an exogenous domain in the adenovirus type 2 fiber globular region.
Insertion of an exogenous domain in the adenovirus type 2 fiber globular region.
复制标题
在 2 型腺病毒纤维球状区域中插入外源结构域。
DOI：
10.1016/s0006-291x(02)02005-3
复制
发表时间：
2002
影响因子：
3.1
通讯作者：
Ventura,AM
中科院分区：
文献类型：
--
作者：
Zerbini,LF;Libermann,TA;Ventura,AM
Adenoviruses have been used for gene therapy or immunization due to their ability to efficiently infect a broad range of cells and tissues. These applications as well as specificity could be improved further by redirecting binding of the virus to specific cell types. In this regard, modification of viral genes encoding coat proteins is an option to achieve improvement in retargeting. In this report, we describe a substitution in the adenovirus type 2 fiber globular region by the 44 amino acid C4 domain of human immunodeficiency virus type 1 gp120. In vitro translation analysis and immunoprecipitation assays show that the incorporation of the C4 domain into the fiber protein does not ablate its trimerization property and demonstrates the availability of the C4 epitope for interaction with monoclonal anti-C4 antibody. The recombinant adenovirus containing this modified fiber was also characterized by immunoprecipitation with the same antibody, showing the viability of such kind of modification.
