Structure of a fucoidan from the brown seaweed Fucus serratus L.
Structure of a fucoidan from the brown seaweed Fucus serratus L.
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DOI：
10.1016/j.carres.2005.11.009
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发表时间：
2006-02-06
影响因子：
3.1
通讯作者：
Usov, AI
中科院分区：
文献类型：
--
作者：
Bilan, MI;Grachev, AA;Usov, AI
组成的摘要研究L -聚焦、硫酸和乙酸的摩尔比例1:1:0.1和少量的木糖、半乳糖被孤立于棕色海藻墨L .摘要研究结构研究了h - 1 d和2 d和c13核磁共振光谱学desulfated de-O-acetylated衍生品以及本地的甲基化分析和desulfated多糖。结果表明，岩藻糖idan具有3-和4-链-l -岩藻糖基交替的支链结构，3)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)- α -l - fucp -(1 ->)。还检测到由4-连接的α -岩藻糖和β -木糖残基组成的小链，但它们的位置以及半乳糖残基的位置仍然未知。硫酸盐基团主要占据C-2，有时占据C-4，尽管3,4-二糖基化和一些末端聚焦残基可能是非硫酸盐化的。发现乙酸占据3-链Fuc的C-4和4-链Fuc的C-3的比例约为7:3。(c) 2005 Elsevier Ltd版权所有。
A fucoidan consisting Of L-fucose, sulfate and acetate in a molar proportion of 1:1:0.1 and small amounts of xylose and galactose were isolated from the brown seaweed Fucus serratus L. The fucoidan structure was investigated by 1D and 2D H-1 and C-13 NMR spectroscopy of its desulfated and de-O-acetylated derivatives as well as by methylation analysis of the native and desulfated polysaccharides. A branched structure was suggested for the fucoidan with a backbone of alternating 3- and 4-linked alpha-L-fucopyranose residues, 3)-alpha-L-Fucp-(1 -> 4)-alpha-L-Fucp-(1 -> about half of the 3-linked residues being substituted at C-4 by trifucoside units alpha-L-Fucp-(1 -> 4)-alpha-L-Fucp-(1 -> 3)-alpha-L-Fucp-(1 ->. Minor chains built up of 4-linked alpha-fucopyranose and beta-xylose residues were also detected, but their location, as well as the position of galactose residues, remained unknown. Sulfate groups were shown to occupy mainly C-2 and sometimes C-4, although 3,4-diglycosylated and some terminal fucose residues may be nonsulfated. Acetate was found to occupy C-4 of 3-linked Fuc and C-3 of 4-linked Fuc in a ratio of about 7:3. (c) 2005 Elsevier Ltd. All rights reserved.