Genome-Wide Identification of the PHD-Finger Family Genes and Their Responses to Environmental Stresses in Oryza sativa L.
Genome-Wide Identification of the PHD-Finger Family Genes and Their Responses to Environmental Stresses in Oryza sativa L.
复制标题
水稻 PHD-finger 家族基因的全基因组鉴定及其对环境胁迫的响应。
DOI：
10.3390/ijms18092005
复制
发表时间：
2017-09-01
影响因子：
5.6
通讯作者：
Sun, Xiaoli
中科院分区：
文献类型：
--
作者：
Sun, Mingzhe;Jia, Bowei;Sun, Xiaoli
PHD指蛋白家族已被证明参与调控植物的生长和发育。然而，关于其在环境胁迫响应中的作用信息却很少。在此，我们在水稻基因组中总共鉴定出59个PHD家族基因。这些OsPHDs基因位于11条染色体上，同线性分析仅揭示出水稻PHD家族内有9对重复基因。对所有OsPHDs以及来自其他物种的PHDs进行的系统发育分析表明，它们可分为两个主要的类群。此外，OsPHDs被聚类为8个组，不同组的成员在基因结构、功能结构域和保守基序方面表现出很大差异。我们还发现，除了OsPHD6外，所有的OsPHDs在10种被检测的组织中的至少一种中表达，并且某些组的OsPHDs在特定组织中表达。此外，我们的研究结果还揭示了OsPHDs的表达对环境胁迫（包括脱落酸（ABA）、水分亏缺、寒冷和高镉）的不同响应。通过使用实时定量PCR，我们进一步证实了在这些胁迫下OsPHDs的差异表达。OsPHD1/7/8/13/33在水分亏缺和镉胁迫下差异表达，而OsPHD5/17在水分亏缺和寒冷胁迫下表达发生改变。此外，OsPHD3/44/28在ABA和镉胁迫下表现出差异表达。总之，我们的研究结果为水稻PHD家族在植物对环境胁迫的响应方面提供了有价值的信息，这将有助于进一步阐明它们在应对环境胁迫中的生物学作用。
The PHD-finger family has been demonstrated to be involved in regulating plant growth and development. However, little information is given for its role in environmental stress responses. Here, we identified a total of 59 PHD family genes in the rice genome. These OsPHDs genes were located on eleven chromosomes and synteny analysis only revealed nine duplicated pairs within the rice PHD family. Phylogenetic analysis of all OsPHDs and PHDs from other species revealed that they could be grouped into two major clusters. Furthermore, OsPHDs were clustered into eight groups and members from different groups displayed a great divergence in terms of gene structure, functional domains and conserved motifs. We also found that with the exception of OsPHD6, all OsPHDs were expressed in at least one of the ten tested tissues and OsPHDs from certain groups were expressed in specific tissues. Moreover, our results also uncovered differential responses of OsPHDs expression to environmental stresses, including ABA (abscisic acid), water deficit, cold and high Cd. By using quantitative real-time PCR, we further confirmed the differential expression of OsPHDs under these stresses. OsPHD1/7/8/13/33 were differentially expressed under water deficit and Cd stresses, while OsPHD5/17 showed altered expression under water deficit and cold stresses. Moreover, OsPHD3/44/28 displayed differential expression under ABA and Cd stresses. In conclusion, our results provide valuable information on the rice PHD family in plant responses to environmental stress, which will be helpful for further characterizing their biological roles in responding to environmental stresses.