Circular dichroism of quadruplex DNAs: Applications to structure, cation effects and ligand binding
Circular dichroism of quadruplex DNAs: Applications to structure, cation effects and ligand binding
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DOI：
10.1016/j.ymeth.2007.02.009
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发表时间：
2007-12-01
期刊：
影响因子：
4.8
通讯作者：
Bolton, Philip H.
中科院分区：
文献类型：
--
作者：
Paramasivan, Sattanathan;Rujan, Iulian;Bolton, Philip H.
圆二色性（CD）光谱可用于获得DNA四链体结构的信息以及序列、阳离子、化学修饰和配体结合对四链体结构的影响。目前还没有一种有效的方法来计算CD光谱从一个四链体结构，也不是他们的一个去从CD光谱的结构。然而，在许多情况下，可以凭经验将CD光谱特征与四链体结构类型相关联。在这篇文章中，四个案例研究表明CD在端粒重复序列形成的四链体结构的性质的调查的优势和局限性。案例研究包括确定四链体结构类型作为钾浓度的函数，序列对四链体结构类型之间平衡的影响作为钾浓度的函数，配体结合对四链体结构的影响和5'磷酸化对四链体结构类型的影响。(c)2007年爱思唯尔公司All rights reserved.
Circular dichroism, CD, spectra can be used to gain information about quadruplex structures of DNAs as well as the effects of sequence, cations, chemical modification and ligand binding on quadruplex structure. There is not yet a validated approach to calculate a CD spectrum from a quadruplex structure nor is their one to go from a CD spectrum to a structure. However, it is possible to empirically correlate CD spectra features with quadruplex structural type in many cases. In this article four case studies are presented to indicate the strengths and limitations of CD in investigations of the properties of quadruplex structures formed by telomere repeat sequences. The case studies include determination of the quadruplex structural type present as a function of potassium concentration, the effect of sequence on the equilibrium between quadruplex structural types as a function of potassium concentration, the effect of ligand binding on quadruplex structure and the effect of 5' phosphorylation on quadruplex structural type. (c) 2007 Elsevier Inc. All rights reserved.