[Prostate specific monoclonal antibody gained by glycolipid immunization].
[Prostate specific monoclonal antibody gained by glycolipid immunization].
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糖脂免疫获得前列腺特异性单克隆抗体[J].
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Y. Hashimoto
中科院分区：
文献类型：
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作者：
M. Satoh;Y. Fukushi;C. Oyama;S. Saitoh;S. Orikasa;T. Tochigi;K. Sugawara;T. Masuko;Y. Hashimoto
分别用氯仿-甲醇溶液和异丙醇-己烷溶液从前列腺增生组织和前列腺癌组织中提取糖脂成分。采用Folchs分级技术将提取物分离为“上相”和“下相”。将每一级分与酸处理的saltingminesota缀合，然后将其皮下、腹膜内和最后静脉内注射至BALB/c小鼠。将免疫小鼠的脾细胞与P3 X63融合，获得抗体APG 1。免疫组化分析显示，APG 1对前列腺组织具有优先反应性。此外，酶和酸处理组织的免疫组织化学分析和TLC免疫染色表明，该单克隆抗体特异性识别前列腺组织中表达的唾液酸糖链。
Extraction of glycolipid component from hypertrophic and cancerous prostatic tissue were performed using chloroform-methanol solution and isopropanol-hexan-solution. The extract was separated into the "upper phase" and "lower phase" by Folchs fractionation technic. Each fraction was conjugated with acid treated salmonella minesota and than it was injected subcutaneously, intra-peritoneally and finally intravenously to BALB/c mice. Spleen cells from immunized mice were fused with P3X63, and antibody APG1 was obtained. APG1 shows a preferential reactivity towards prostatic tissue by the immunohistochemical analysis. Furthermore, the immunohistochemical analysis using enzyme and acid treated tissue and TLC immunostaining indicate that this monoclonal antibody specifically recognizes the sialylglycochain expressed in the prostatic tissue.
神经节苷脂：结构、分离和分析。
影响因子：
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作者：
Ledeen,RW;Yu,RK
通讯作者：
Yu,RK