Monitoring of Cadmium influence on ultra short-term growth dynamics of plants using a highly sensitive interferometric technique, SIT
Monitoring of Cadmium influence on ultra short-term growth dynamics of plants using a highly sensitive interferometric technique, SIT
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使用高灵敏度干涉技术 SIT 监测镉对植物超短期生长动态的影响
DOI：
10.1117/12.2196866
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hirofumi Kadono
中科院分区：
文献类型：
--
作者：
Kokge T. Desilva;Hirofumi Kadono
镉（Cd）是一种具有高毒性的环境重金属污染物。本研究的目的是研究镉对植物生长动力学的影响在亚纳米级，使用一种新的光学干涉技术，命名为统计干涉技术（SIT）。在这项研究中，一个特别注意的是，在测量叶片的面内位移的短期生长波动。以韭菜为试材，研究了镉暴露对韭菜生长及其纳米生长波动的影响。在我们之前的研究中发现的这种纳米波动是植物的固有特性，被称为纳米固有波动（NIF）。Cd对植物生长波动的影响，用0、0.001、0.01和0.1mM四种浓度的CdCl_2溶液连续处理三天，观察其生长速率的NIF。健康叶片NIF的标准差为4.0nm/mm sec，在0.1mM Cd处理6 h和54 h后分别降至3.1nm/mm sec和1.8nm/mm sec。对于0.01mM的较小浓度，与0.1mM相比，证实NIF的SD降低较少。此外，在0.001mM下，在前6小时内快速降低后可以观察到显著的恢复。结果表明，NIF可以作为重金属胁迫的一种检测手段，并且能够在很早的阶段检测到较少量的Cd（从0.001mM到0.1mM）对植物的影响。
Cadmium(Cd) is an environmental contaminant heavy metal having high toxicity. The aim of this study is to investigate the effect of Cd on growth dynamics of plants in the order of sub-nanometers, using a novel optical interference technique, named as Statistical Interferometry Technique(SIT). In this study, a special attention is paid to the short-term growth fluctuation in measurements of the in-plane displacement of the leaf. In the experiments, Chinese chives(Allium Tuberosum) were used as samples, and the growth and its nanometric growth fluctuations were measured for Cd exposure. This nanometric fluctuation that was found in our previous study, is an intrinsic property of the plant and is referred to as nanometric intrinsic fluctuations(NIF). The effect of Cd on plant growth fluctuation, i.e., NIF of growth rate was observed for three days continuously by exposing their roots to four CdCl2concentrations 0, 0.001, 0.01, and 0.1mM. The standard deviation(SD) of NIF of healthy leaf was 4.0nm/mm sec, and it reduced to 3.1nm/mm sec and 1.8nm/mm sec after 6 hours and 54 hours after exposing to 0.1mM Cd, respectively. For smaller concentration of 0.01mM, less reduction in SD of NIF was confirmed compared to those for 0.1mM. In addition, under 0.001mM, a significant recovery could be observed after a rapid reduction in the first 6 hours. The results imply that NIF can be a measure for heavy metal stress and is sensitive enough to detect the influence of smaller amount of Cd(from 0.001mM to 0.1mM) on plants in a very early stage.