Thermal Discrimination and Transgenerational Temperature Response in Bemisia tabaci Mediterranean (Hemiptera: Aleyrodidae): Putative Involvement of the Thermo-Sensitive Receptor BtTRPA
Thermal Discrimination and Transgenerational Temperature Response in Bemisia tabaci Mediterranean (Hemiptera: Aleyrodidae): Putative Involvement of the Thermo-Sensitive Receptor BtTRPA
复制标题
地中海烟粉虱（半翅目：粉虱科）的热辨别和跨代温度响应：热敏受体 BtTRPA 的推定参与
DOI：
10.1093/ee/nvx202
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发表时间：
2018-02-01
影响因子：
1.7
通讯作者：
Wan, Fang-Hao
中科院分区：
文献类型：
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作者：
Dai, Tian-Mei;Wang, Yu-Sheng;Wan, Fang-Hao
摘要人为气候变化和全球变暖预计将改变许多外生物种的地理分布和丰度，这将增加外来物种对新地区的入侵。为了在多变或波动的温度条件下生存，昆虫需要敏感的热感觉机制来检测外部热刺激并诱导适当的行为和生理反应。TRPA是一种热激活的瞬时受体电位(Trp)家族离子通道，对昆虫的趋热性是必不可少的。在这里，我们研究了BtTRPA在地中海烟粉虱短期和长期热应激中的潜在作用。我们发现BtTRPA主要在触角所在的头部表达。在短期热应激条件下，BtTRPA基因在急性低温和高温暴露后均有较强的表达，0℃暴露3h和40℃暴露5h达到最高水平，但在较温和的刺激(12℃和35℃)下表达相对较低。这些结果表明，BtTRPA可以区分无害和有害的温度刺激。在长期热胁迫下，BtTRPA的表达水平在21℃和31℃温和无害温度下的G1时达到最高，BtTRPA在成年雌性中急剧增加并达到峰值，这表明长期温和无害的热暴露可能会产生跨代表达效应，从而增强后代应对相同胁迫的能力。本研究证明了BtTRPA通道在温度敏感中的重要作用，并为适应地中海烟粉虱不同环境温度的温度调节提供了分子基础。
Abstract Anthropogenic climate change and global warming are expected to alter the geographic distribution and abundance of many ectothermic species, which will increase the invasion of new areas by exotic species. To survive in variable or fluctuating temperature conditions, insects require sensitive thermal sensory mechanisms to detect external thermal stimuli and induce the appropriate behavioral and physiological responses. TRPA, a thermal-activated transient receptor potential (TRP) family ion channel, is essential for thermotaxis in insects. Here, we investigated the potential role of BtTRPA in short-term and long-term thermal stress in Bemisia tabaci Mediterranean (Gennadius; Hemiptera: Aleyrodidae). We found that BtTRPA was mainly expressed in the head, where the antennae are located. Under short-term thermal stress, the BtTRPA gene was robustly expressed after exposure to acute low or high temperatures, BtTRPA expression reached the highest levels after exposure to 0°C for 3 h and 40°C for 5 h, but was relatively low after exposure to milder stimuli (12 and 35°C). These results demonstrated that BtTRPA could discriminate between innocuous and noxious temperature stimuli. Under long-term thermal stress, the highest expression level of BtTRPA occurred at G1 exposed to mild innocuous temperature of 21 and 31°C, along with BtTRPA sharply increased and peaked in adult females, implying that mild innocuous long-term thermal exposure could cause transgenerational expression effects to enhance the ability of offspring to cope with the same stress. This study demonstrates that the channel BtTRPA is important in temperature sensing and provides a molecular basis for thermosensation regulation in response to varied environmental temperature in B. tabaci Mediterranean.