Use of collagen shields containing amphotericin B in the treatment of experimental Candida albicans-induced keratomycosis in rabbits.
Use of collagen shields containing amphotericin B in the treatment of experimental Candida albicans-induced keratomycosis in rabbits.
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DOI：
10.1016/s0002-9394(14)71583-1
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发表时间：
1992-03
影响因子：
4.2
通讯作者：
U. Pleyer;A. Legmann;B. Mondino;D. Lee
中科院分区：
文献类型：
--
作者：
U. Pleyer;A. Legmann;B. Mondino;D. Lee
我们评价了用白念珠菌素B预浸的胶原膜对实验性白念珠菌引起的角膜炎的治疗效果。将治疗结果与每小时滴入的阿替霉素B滴眼液的治疗结果进行比较。48只白化病兔接受108 C基质内注射。白色微生物24小时后，每天用每小时滴注0.15%阿替霉素B滴剂、每小时滴注生理盐水滴剂或应用在0.5%阿替霉素B中预浸泡1小时的胶原蛋白护罩治疗眼睛8小时。处理1、3或5天后处死家兔。通过培养匀浆和计数菌落形成单位来定量角膜中的真菌。与生理盐水处理的对照眼相比，每小时滴注或吸收在胶原膜中的阿替霉素B治疗在所有时间点均显著降低角膜真菌计数（P <0.05）。在第1天（P = 0.02）和第3天（P = 0.04），与每小时滴注一次阿替霉素B滴剂相比，用阿替霉素B浸泡的胶原蛋白护罩治疗的兔眼真菌计数显著更低，但在第5天并非如此。在第5天，胶原蛋白屏障在减少集落形成单位的数量方面与阿替霉素B滴剂一样有效。这些数据表明，浸在阿替霉素B中的胶原膜可能是一种有用和方便的治疗角膜真菌病的装置，例如由C引起的角膜真菌病。白色念珠菌。
We evaluated the effect of collagen shields presoaked with amphotericin B on the treatment of experimentalCandida albicans-induced keratitis. Treatment results were compared to those of amphotericin B eyedrops instilled hourly. Forty-eight albino rabbits received intrastromal injections of 108C. albicansorganisms. Twenty-four hours later, eyes were treated for eight hours each day with hourly instillation of 0.15% amphotericin B drops, hourly instillation of saline drops, or application of a collagen shield presoaked in 0.5% amphotericin B for one hour. The rabbits were killed after one, three, or five days of treatment. Quantitation of fungi in the cornea was achieved by culturing homogenates and counting colony-forming units. Treatment with amphotericin B applied either as hourly instilled drops or absorbed in collagen shields significantly (P < .05) reduced corneal fungal counts at all time points when compared to saline-treated control eyes. Rabbit eyes treated with amphotericin B-soaked collagen shields had significantly lower fungal counts compared with hourly instilled amphotericin B drops at Days 1 (P = .02) and 3 (P = .04), but not at Day 5. The collagen shields were as effective in reducing the number of colony-forming units as were amphotericin B drops at Day 5. These data suggest that collagen shields soaked in amphotericin B could be a useful and convenient treatment device in keratomycosis such as that caused byC. albicans.