Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure.
Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure.
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DOI：
10.3389/fmicb.2021.696730
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wu R
中科院分区：
文献类型：
--
作者：
Yang D;Zheng X;Jiang L;Ye M;He X;Jin Y;Wu R
Phenotypic plasticity is the exhibition of various phenotypic traits produced by a single genotype in response to environmental changes, enabling organisms to adapt to environmental changes by maintaining growth and reproduction. Despite its significance in evolutionary studies, we still know little about the genetic control of phenotypic plasticity. In this study, we designed and conducted a genome-wide association study (GWAS) to reveal genetic architecture of how Staphylococcus aureus strains respond to increasing concentrations of vancomycin (0, 2, 4, and 6 μg/mL) in a time course. We implemented functional mapping, a dynamic model for genetic mapping using longitudinal data, to map specific loci that mediate the growth trajectories of abundance of vancomycin-exposed S. aureus strains. 78 significant single nucleotide polymorphisms were identified following analysis of the whole growth and development process, and seven genes might play a pivotal role in governing phenotypic plasticity to the pressure of vancomycin. These seven genes, SAOUHSC_00020 (walR), SAOUHSC_00176, SAOUHSC_00544 (sdrC), SAOUHSC_02998, SAOUHSC_00025, SAOUHSC_00169, and SAOUHSC_02023, were found to help S. aureus regulate antibiotic pressure. Our dynamic gene mapping technique provides a tool for dissecting the phenotypic plasticity mechanisms of S. aureus under vancomycin pressure, emphasizing the feasibility and potential of functional mapping in the study of bacterial phenotypic plasticity.
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影响因子：
5.2
作者：
He X;Jin Y;Ye M;Chen N;Zhu J;Wang J;Jiang L;Wu R
通讯作者：
Wu R
影响因子：
5.2
作者：
Buchanan CJ;Webb AL;Mutschall SK;Kruczkiewicz P;Barker DOR;Hetman BM;Gannon VPJ;Abbott DW;Thomas JE;Inglis GD;Taboada EN
通讯作者：
Taboada EN
影响因子：
3.2
作者：
Baseri, Neda;Najar-Peerayeh, Shahin;Bakhshi, Bita
通讯作者：
Bakhshi, Bita
绘制控制器官生长和全株生物量的协变数量性状位点
影响因子：
5.6
作者：
Gan, Jingwen;Cao, Yige;Wu, Rongling
通讯作者：
Wu, Rongling
通过实施高通量表型分析，对小麦产量组成性状中氮缺乏诱导的反应进行功能图谱
影响因子：
7.2
作者：
Jiang, Libo;Sung, Lidan;Wu, Rongling
通讯作者：
Wu, Rongling