Efficient method for establishing induced pluripotent stem cells
Efficient method for establishing induced pluripotent stem cells
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发表时间：
2008-10
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通讯作者：
겐이치 데즈카;도시유키 시바타;다카히로 구니사다;나리타카 다마오키;도모코 다케다;신야 야마나카;가즈토시 다카하시
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作者：
겐이치 데즈카;도시유키 시바타;다카히로 구니사다;나리타카 다마오키;도모코 다케다;신야 야마나카;가즈토시 다카하시
本发明提供了一种产生诱导多能干（iPS）细胞的方法，其包括使核重编程材料与干细胞的尺寸接触。通过使用一维干细胞作为体细胞的来源，可以通过三种或四种因子的转移显著提高人iPS细胞的建立效率。此外，维干细胞可以从提取物中分离和制备，因为这样提取的智齿和牙周病牙，这是因为它可以广泛用作iPS细胞库的体细胞来源，容易获得。
The present invention provides a method of producing, induced pluripotent stem (iPS) cell comprising contacting the nuclear reprogramming material and the dimensions of stem cells. By using a dimension stem cells as a source of somatic cells, it is possible to remarkably improve the efficiency of establishment of human iPS cells by the transfer of three or four factors. In addition, the dimensions stem cells can be isolated and prepared from the extracted because such extracted wisdom tooth and periodontal disease, tooth, which because it can be used extensively as a source of somatic cells for iPS cell bank, readily available.