Hepatitis E Virus of Subtype 3a in a Pig Farm, South-Eastern France
Hepatitis E Virus of Subtype 3a in a Pig Farm, South-Eastern France
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DOI：
10.1111/zph.12211
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发表时间：
2015-12-01
影响因子：
2.4
通讯作者：
Davoust, B.
中科院分区：
文献类型：
--
作者：
Colson, P.;St-Jacques, P.;Davoust, B.
戊型肝炎病毒(HEV)是近十年来出现的一种地方性肝炎的病原体，在西方发达国家引起临床关注。越来越多的人认识到，猪是全球3和4型HEV的主要储备者，而猪衍生食品是这些病毒在人类中感染的潜在来源。这里对2012年从50头3个月大的猪场采集的直肠拭子的粪便进行了戊型肝炎病毒RNA检测，这些猪场位于法国东南部，与2007年进行的前一项工作不同。通过室内RT-PCR扩增获得了与ORF2和ORF1基因片段相对应的猪HEV基因序列。通过系统发育分析确定戊型肝炎病毒的基因分型。患病率与5年前测定的结果相似(68%对62%)。鉴定了HEV 3a和3f亚型的两个强大的系统发育簇，2012年获得的这些序列与2007年获得的序列有很大差异。值得注意的是，2012年从大多数(62%)受感染的猪身上获得的HEV序列属于3a亚型，这在法国以前没有描述过，包括在任何人、猪或野猪中都没有发现。需要进一步的研究来评估HEV-3a在这个国家的猪和人中的传播情况。此外，与以前的研究结果一起，这项研究支持需要向公众提供更多关于通过接触猪或在法国消费猪衍生产品而感染HEV的风险的信息。
Hepatitis E virus (HEV) has emerged during the past decade as a causative agent of autochthonous hepatitis and is a clinical concern in Western developed countries. It has been increasingly recognized that pigs are a major reservoir of HEV of genotypes 3 and 4 worldwide and pig-derived food items represent a potential source of infections by these viruses in humans. Hepatitis E virus RNA testing was performed here on faeces from rectal swabs sampled in 2012 from 50 3-month-old farm pigs from the same farm located in south-eastern France than in a previous work conducted in 2007. Pig HEV sequences corresponding to genomic fragments of ORF2 and ORF1 genes were obtained after RT-PCR amplification with in-house protocols. Hepatitis E virus genotype was determined by phylogenetic analysis. Prevalence was similar to that determined 5 years earlier (68% versus 62%). Two robust phylogenetic clusters of HEV subtypes 3a and 3f were identified, and these sequences obtained in 2012 largely differ compared with those obtained in 2007. Notably, HEV sequences obtained in 2012 from a majority (62%) of the infected pigs belonged to subtype 3a, which was not previously described in France, including not being found in any of humans, pigs or wild boars. Further studies are needed to assess the circulation of HEV-3a in pigs and humans in this country. In addition, along with previous findings, this study supports the need for increased information to the public on the risk of HEV infection through contacts with pigs or consumption of pig-derived products in France.