Biogenic and chemogenic synthesis of TiO2 NPs via hydrothermal route and their antibacterial activities
Biogenic and chemogenic synthesis of TiO2 NPs via hydrothermal route and their antibacterial activities
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DOI：
10.1039/c6ra22163g
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Sidarai, Ashok H.
中科院分区：
文献类型：
--
作者：
Hunagund, Shirajahammad M.;Desai, Vani R.;Sidarai, Ashok H.
在此，我们概述了一种简单的水热路线，使用新型生物源蒌叶提取物和化学源硝酸作为封端剂和还原剂来合成二氧化钛（TiO2）纳米颗粒（NP）。对合成的 TiO2 纳米颗粒进行了各种表征技术，如紫外-可见分光光度法、X 射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR)、透射电子显微镜 (TEM) 和能量色散 X 射线光谱 (EDS)，以进行光学、结构、形态和成分分析。获得的结果揭示了以下特征； XRD 分析证实了纳米晶 TiO2 的相形成和存在。 FT-IR 光谱分析揭示了 Ti-O 振动带、O-H 带、植物蛋白、单宁、碳水化合物和多元醇的存在。 TEM 分析显示，生物合成和化学合成的 TiO2 的纳米粒子均呈球形，平均尺寸分别约为 8 nm 和 75 nm。 EDS 分析证实了具有 Ti 和 O 元素的 NP 的化学成分。此外，还研究了这些合成的 TiO2 NPs 对金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）等多重耐药微生物的抗菌活性；在此我们观察到生物源性和化学源性的最低抑菌浓度（MIC）值分别为25μg·mL(-1)和50μg·mL(-1)。这些结果表明，生物合成的纳米颗粒比化学合成的纳米颗粒作为更有效的抗菌剂。
Herein, we outline a simple hydrothermal route for the synthesis of titanium dioxide (TiO2) nanoparticles (NPs) using novel biogenic source Piper betel leaf extract and chemogenic source nitric acid as capping and reducing agents. The synthesized TiO2 NPs were subjected to various characterization techniques such as UV-vis spectrophotometry, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS) for their optical, structural, morphological and compositional analysis. The obtained results reveal the following features; XRD analysis confirms the phase formation and presence of nanocrystalline TiO2. FT-IR spectroscopy analysis reveals the presence of Ti-O vibrational bands, O-H bands, plant proteins, tannins, carbohydrates and polyols. TEM analysis shows the NPs were of spherical shape with an average size of about 8 nm and 75 nm for biogenic and chemogenic synthesized TiO2 respectively. EDS analysis confirms the chemical compositions of the NPs having Ti and O elements. Further, these synthesized TiO2 NPs were studied for their antibacterial activity against multi-drug resistant microorganisms like Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative); here we observe that the minimum inhibitory concentration (MIC) values for biogenic and chemogenic are 25 mu g mL(-1) and 50 mu g mL(-1) respectively. These results suggest that biogenic synthesized NPs act as a more effective antibacterial agent than chemogenic synthesized NPs.