Transposon tagging using Ty elements in yeast.
Transposon tagging using Ty elements in yeast.
复制标题
使用酵母中的 Ty 元件进行转座子标记。
DOI：
10.1093/genetics/120.1.95
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Strathern,JN
中科院分区：
文献类型：
--
作者：
Garfinkel,DJ;Mastrangelo,MF;Sanders,NJ;Shafer,BK;Strathern,JN
我们已经利用诱导高水平的Ty转座的能力来开发在酿酒酵母中进行转座子诱变的方法。为了便于遗传和分子分析，我们构建了GAL1启动的TyH3或Ty917元件，这些元件含有独特的克隆位点，并标记这些元件与选择基因。这些基因包括酵母HIS 3基因和含有Tn903 NEO基因的质粒PiAN 7。标记的Ty元件保留其转座、突变LYS2、LYS5或STE2基因以及激活无启动子的his3 delta 4靶基因的能力。含有选择基因的Ty元件也可用于菌株构建、染色体作图和基因克隆策略。
We have used the ability to induce high levels of Ty transposition to develop a method for transposon mutagenesis in Saccharomyces cerevisiae. To facilitate genetic and molecular analysis, we have constructed GAL1-promoted TyH3 or Ty917 elements that contain unique cloning sites, and marked these elements with selectable genes. These genes include the yeast HIS3 gene, and the plasmid PiAN7 containing the Tn903 NEO gene. The marked Ty elements retain their ability to transpose, to mutate the LYS2, LYS5, or STE2 genes, and to activate the promoterless his3 delta 4 target gene. Ty elements containing selectable genes are also useful in strain construction, in chromosomal mapping, and in gene cloning strategies.