SUBSTANCE-P - DEPLETION IN THE DORSAL HORN OF RAT SPINAL-CORD AFTER SECTION OF THE PERIPHERAL PROCESSES OF PRIMARY SENSORY NEURONS
SUBSTANCE-P - DEPLETION IN THE DORSAL HORN OF RAT SPINAL-CORD AFTER SECTION OF THE PERIPHERAL PROCESSES OF PRIMARY SENSORY NEURONS
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DOI：
10.1016/0006-8993(79)90167-7
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发表时间：
1979-01-01
期刊：
影响因子：
2.9
通讯作者：
OTSUKA, M
中科院分区：
文献类型：
--
作者：
JESSELL, T;TSUNOO, A;OTSUKA, M
测定了单侧坐骨神经切断或背根切断后大鼠腰髓不同部位的P物质含量、谷氨酸脱羧酶和胆碱乙酰转移酶活性以及[~ 3 H]二丙诺啡结合水平。切断坐骨神经后，脊髓背角P物质含量减少75-80%，而脊髓腹角P物质含量无明显变化。P物质消耗的发作发生在7天内，并维持2个月。坐骨神经切断后，背根神经节和初级感觉神经元的外周支和中央支的P物质含量也减少。谷氨酸脱羧酶和胆碱乙酰转移酶活性不变，阿片受体结合发生1个月的小幅度下降。神经切断后。背根切断术产生了80%的P物质在背角耗尽。腹角P物质含量显著降低。背根切断术不影响背角谷氨酸脱羧酶活性，而阿片受体结合减少了40%。周围神经损伤导致初级感觉神经元变性，这些神经元含有并释放作为神经递质的P物质。
The substance P content, glutamic acid decarboxylase and choline acetyltransferase activities and the level of [3H]diprenorphine binding were measured in various regions of the lumbar spinal cord of rats after unilateral section of the sciatic nerve or after dorsal rhizotomy. Sciatic nerve section produced a 75-80% depletion of substance P in the dorsal horn but did not change the substance P content of the ventral horn. The onset of substance P depletion occurred within 7 days and was maintained for 2 mo. The substance P content of the dorsal root ganglia and the peripheral and central branches of primary sensory neurons was also reduced after sciatic nerve section. Glutamic acid decarboxylase and choline acetyltransferase activity were unchanged; a small decrease in opiate receptor binding occurred 1 mo. after nerve section. Dorsal rhizotomy produced an 80% depletion of substance P in the dorsal horn. The substance P content of the ventral horn was significantly reduced. Glutamic acid decarboxylase activity in the dorsal horn was unaffected by dorsal rhizotomy, whereas opiate receptor binding was reduced by 40%. Peripheral nerve injury results in the degeneration of primary sensory neurons which contain and release substance P as neurotransmitter.