EXPRESSION OF FASCICLIN-I AND FASCICLIN-II GLYCOPROTEINS ON SUBSETS OF AXON PATHWAYS DURING NEURONAL DEVELOPMENT IN THE GRASSHOPPER
EXPRESSION OF FASCICLIN-I AND FASCICLIN-II GLYCOPROTEINS ON SUBSETS OF AXON PATHWAYS DURING NEURONAL DEVELOPMENT IN THE GRASSHOPPER
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DOI：
10.1016/0092-8674(87)90072-9
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发表时间：
1987-03-13
期刊：
影响因子：
64.5
通讯作者：
GOODMAN, CS
中科院分区：
文献类型：
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作者：
BASTIANI, MJ;HARRELSON, AL;GOODMAN, CS
“标记通路”假说预测胚胎神经元中的轴突束被用于生长锥引导的表面识别分子差异标记。为了确定候选人，这样的识别分子，我们产生的单克隆抗体（MAbs），识别表面抗原表达的轴突束的蝗虫胚胎的子集。3B 11和8 C6单克隆抗体免疫沉淀70-和95-kd膜糖蛋白，分别称为fasciclin I和II，这是在不同子集的轴突束在发展过程中表达。这两种糖蛋白在胚胎轴突的特定部分上区域性表达，与它们的成束模式相关，在轴突生长期间以与生长锥指导作用一致的方式动态地表达，并且在胚胎发生期间的其他时间和地点表达，表明多种发育作用。
The "labeled pathways" hypothesis predicts that axon fascicles in the embryonic neuropil are differentially labeled by surface recognition molecules used for growth cone guidance. To identify candidates for such recognition molecules, we generated monoclonal antibodies (MAbs) that recognize surface antigens expressed on subsets of axon fascicles in the grasshopper embryo. The 3B11 and 8C6 MAbs immunoprecipitate 70- and 95-kd membrane glycoproteins called fasciclin I and II, respectively, which are expressed on different subsets of axon fascicles during development. These two glycoproteins are expressed regionally on particular portions of embryonic axons in correlation with their patterns of fasciculation, dynamically during the period of axon outgrowth in a manner consistent with a role in growth cone guidance, and at other times and places during embryogenesis, suggesting multiple developmental roles.