MITOCHONDRIAL-DNA VARIATION IN CHINOOK (ONCORHYNCHUS-TSHAWYTSCHA) AND CHUM SALMON (O KETA) DETECTED BY RESTRICTION ENZYME ANALYSIS OF POLYMERASE CHAIN-REACTION (PCR) PRODUCTS
MITOCHONDRIAL-DNA VARIATION IN CHINOOK (ONCORHYNCHUS-TSHAWYTSCHA) AND CHUM SALMON (O KETA) DETECTED BY RESTRICTION ENZYME ANALYSIS OF POLYMERASE CHAIN-REACTION (PCR) PRODUCTS
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DOI：
10.1139/f93-081
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发表时间：
1993-04-01
影响因子：
2.4
通讯作者：
BICKHAM, JW
中科院分区：
文献类型：
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作者：
CRONIN, MA;SPEARMAN, WJ;BICKHAM, JW
我们分析了来自育空河(阿拉斯加和育空地区)、基奈河(阿拉斯加)、俄勒冈河和加利福尼亚州河流的Chinook鲑鱼(Oncorhynchus Tshawytscha)以及来自育空河、温哥华岛和华盛顿河的chum鲑鱼(O.keta)的种内线粒体DNA变异。对于每个物种，分别使用聚合酶链式反应扩增mtDNA分子的三个不同部分，然后用至少19种限制性内切酶进行消化。单倍型之间的种内序列差异小于每核苷酸0.01个碱基替换。共鉴定出9种鲑鱼单倍型。育空河鲑鱼种群显示出更多的单倍型(8个)，比温哥华岛和华盛顿的种群(2个)更多。最常见的鲑鱼单倍型出现在所有地区。共鉴定出7个Chinook鲑鱼单倍型。育空河和基奈河有四种单倍型，俄勒冈州/加利福尼亚州有四种，只有一种单倍型在这两个地区之间共享。样本量太小，无法量化不同排水系统之间的种群分离程度，但我们观察到的变异模式表明，该技术在遗传种群鉴定中具有实用价值。
We analyzed intraspecific mitochondrial DNA variation in chinook salmon (Oncorhynchus tshawytscha) from drainages in the Yukon River (Alaska and Yukon Territory), the Kenai River (Alaska), and Oregon and California rivers; and chum salmon (O. keta) from the Yukon River and Vancouver Island, and Washington rivers. For each species, three different portions of the mtDNA molecule were amplified separately using the polymerase chain reaction and then digested with at least 19 restriction enzymes. Intraspecific sequence divergences between haplotypes were less than 0.01 base substitution per nucleotide. Nine chum salmon haplotypes were identified. Yukon River chum salmon stocks displayed more haplotypes (eight) than the stocks of Vancouver Island and Washington (two). The most common chum salmon haplotype occurred in all areas. Seven chinook salmon haplotypes were identified. Four haplotypes occurred in the Yukon and Kenai rivers and four occurred in Oregon/California, with only one haplotype shared between the regions. Sample sizes were too small to quantify the degree of stock separation among drainages, but the patterns of variation that we observed suggest utility of the technique in genetic stock identification.