The Comparison of Temporal Transcriptome Changes Between Morning-Opening and Afternoon-Opening Iris Flowers Reveals the Candidate Genes Regulating Flower Opening and Closing
The Comparison of Temporal Transcriptome Changes Between Morning-Opening and Afternoon-Opening Iris Flowers Reveals the Candidate Genes Regulating Flower Opening and Closing
复制标题
DOI：
10.1007/s12374-022-09363-4
复制
发表时间：
2022-08-13
影响因子：
2.9
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
--
作者：
Liu, Rong;Gao, Yike;Zhang, Qixiang
生物钟使植物能够使其花朵的开放和关闭节奏与日常环境变化同步，以提高生育力和适应性。鸢尾花属Iris dichotoma，I.紫茎泽兰和它们的杂种是短命的，并提出了强大的开放和关闭的节奏。为了揭示调控花开闭的关键基因，比较了鸢尾与野鸢尾F2代在同一时间进程中不同开花过程中转录组的变化。有分歧的花开放和关闭的时间。植物钟相关基因phytochrome-interactingfactor 4-like在花开始开放期间特异性下调，在花开始关闭期间特异性上调。促进生长素合成、生长素分泌、生长素信号传导、细胞吸水、细胞壁松弛和渗透压增加的基因在花开放初期特异性上调，表明它们对花开放初期起着正调控作用。烟草中生长素反应基因SMALL AUXIN UP-REGULATED RNA 64-like的过量表达促进了烟草的开花。在花开始闭合过程中，促进细胞壁合成和松弛、细胞吸水和光合调节的基因特异性下调，而促进细胞壁和蛋白质降解的基因特异性上调。这些结果有助于理解花开放和关闭的调控网络。
The circadian clock allows plants to synchronize their flower opening and closing rhythms with daily environmental changes to enhance fertility and adaptability. Flowers of Iris dichotoma, I. domestica and their hybrids are ephemeral and present robust opening and closing rhythms. To reveal the key genes regulating flower opening and closing, transcriptome changes during the same time course but different flowering processes were compared between two F-2 hybrid plants of Iris dichotoma and I. domestica with divergent flower opening and closing times. Clock-related gene PHYTOCHROME-INTERACTING FACTOR 4-like was specifically down-regulated during flower starting opening and was specifically up-regulated during flower starting closing. Genes promoting auxin synthesis, auxin efflux, auxin signaling, cellular water uptake, cell wall loosening and the increase of osmotic pressure were specifically up-regulated during flower starting opening, suggesting their positive regulation on the start of a flower opening. The overexpression of auxin-responsive gene SMALL AUXIN UP-REGULATED RNA 64-like in tobacco promoted flower opening of tobacco. During flower starting closing, genes promoting cell wall synthesis and loosening, cellular water uptake and osmoregulation were specifically down-regulated, while genes promoting the degradation of cell wall and protein were specifically up-regulated. The results improve the understanding of regulatory networks of flower opening and closing.