Characterization and Map-Based Cloning of the Novel Rice Yellow Leaf Mutantyl3
Characterization and Map-Based Cloning of the Novel Rice Yellow Leaf Mutantyl3
复制标题
新型水稻黄叶突变体 3 的表征和图位克隆
DOI：
10.1007/s12374-020-09275-1
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发表时间：
2021
影响因子：
2.9
通讯作者：
Zhang Ji
中科院分区：
文献类型：
--
作者：
Liu Xi;Huang QianQian;Yang YanRong;Tang Jiayu;Zhao Yanan;Zhang Ji
叶绿素（Chl）是光合生物中获取光能的重要分子。我们分离到一个水稻叶绿素缺失突变体dyl3，该突变体叶片黄化，叶绿素含量降低，叶绿体发育异常。定位克隆结果表明YL3编码镁螯合酶亚基OsCHLH。Inyl3是一个高度保守的氨基酸残基，其C端发生一个氨基酸替换，YL3在所有组织中组成型表达。与野生型相比，yl3对低温敏感。叶绿素生物合成、光合作用和叶绿体发育基因的表达水平在13号染色体上被强烈降低。这些结果为YL3调控水稻叶绿体发育和Chl合成提供了新的见解。
Chlorophyll (Chl) is an essential molecule for harvesting light energy in photosynthetic organisms. We isolatedyellow leaf 3(yl3), a rice Chl-deficient mutant, which showed yellow leaves with decreased Chl content and abnormal chloroplast development. Positional cloning revealed thatYL3encodesOsCHLH, a subunit of magnesium-chelatase. Inyl3, one amino acid substitution occurs at the C-terminal region of a highly conserved amino acid residue.YL3is constitutively expressed in all tissues. Compared with the wild type,yl3was found to be sensitive to low temperature. The expression levels of genes for Chl biosynthesis, photosynthesis and chloroplast development were strongly reduced inyl3. These results provide novel insights regarding how rice chloroplast development and Chl synthesis are regulated byYL3.