Elevated Level of Circulating Platelet-derived Microparticles in Oral Cancer
Elevated Level of Circulating Platelet-derived Microparticles in Oral Cancer
复制标题
口腔癌中循环血小板衍生微粒水平升高
DOI：
10.1177/0022034515592593
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发表时间：
2016-01-01
影响因子：
7.6
通讯作者：
Liu, B.
中科院分区：
文献类型：
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作者：
Ren, J. G.;Man, Q. W.;Liu, B.
许多研究已经证明，循环微粒（MP）在多种疾病（例如，动脉粥样硬化，高血压和糖尿病），但循环MP和口腔鳞状细胞癌（OSCC）之间的关联仍然很大程度上未知。在本研究中，63例口腔鳞癌患者，22例感染性角化囊性牙源性肿瘤患者和31名健康志愿者的循环血小板衍生的MP（PMPs）的特点和定量流式细胞术分析。对口腔鳞癌患者的凝血功能进行相应的评价。同时采用酶联免疫吸附法（ELISA）和免疫组化法检测血浆和肿瘤组织中炎症相关细胞因子的表达。结果表明，口腔鳞癌患者血浆PMPs水平显著高于健康志愿者和感染性角化囊性牙源性肿瘤患者，且与纤维蛋白原水平呈正相关。此外，MP加入到无MP血浆中后，凝血时间显著缩短。OSCC患者血浆和肿瘤组织中IL-6和TNF-α水平显著升高，且与循环中PMPs水平升高密切相关。综上所述，本研究表明，循环中的PMPs水平升高，显示与炎症相关因子的分泌密切相关，可能有助于增加促凝活性在口腔鳞癌患者。
Numerous studies have demonstrated that circulating microparticles (MPs) play important roles in a variety of diseases (e.g., atherosclerosis, hypertension, and diabetes), but the association between circulating MPs and oral squamous cell carcinoma (OSCC) remains largely unknown. In the present study, the circulating platelet-derived MPs (PMPs) in 63 patients with OSCC, 22 patients with infected keratocystic odontogenic tumor, and 31 healthy volunteers were characterized and quantified by flow cytometric analysis. The coagulation function of patients with OSCC was correspondingly evaluated. Meanwhile, the inflammation-related cytokines were detected in plasma by enzyme-linked immunosorbent assay and in tumor tissues by immunohistochemistry. Our results showed that the plasma level of circulating PMPs was significantly higher in OSCC patients compared with healthy volunteers and patients with infected keratocystic odontogenic tumor, and they showed positive correlation with the increased level of fibrinogen. Moreover, the coagulation time was significantly shorter after the MPs were added to the MP-free plasma. Most important, the levels of interleukin 6 and tumor necrosis factor α in plasma and tumor tissues were significantly increased in OSCC patients, which were closely correlated with the elevated level of circulating PMPs. In summary, this study suggests that the elevated level of circulating PMPs, showing close correlation with the secretion of inflammation-related factors, may contribute to the increased procoagulant activity in patients with OSCC.