Isolation of a peptide fraction from honeybee royal jelly as a potential antifoulbrood factor
Isolation of a peptide fraction from honeybee royal jelly as a potential antifoulbrood factor
复制标题
DOI：
10.1051/apido:2001129
复制
发表时间：
2001-05-01
期刊：
影响因子：
2.4
通讯作者：
Simúth, J
中科院分区：
文献类型：
--
作者：
Bíliková, K;Wu, GS;Simúth, J
以蜂王浆为原料，在酸性条件下进行双重透析，分离得到肽段。主要肽的n端氨基酸序列为V-T-C-D-L-L-S-F-K-G。这一序列对应于蜜蜂防御素王氏蛋白的MW 5523 Da，已被证明对一些革兰氏阳性细菌具有抗菌活性。琼脂平板上的扩散试验表明，肽段对蜜蜂病原体Paenibacillus幼虫（美国褐变病的主要病原体）以及其他革兰氏阳性细菌（如枯草芽孢杆菌和黄斑肌杆菌）有抑制作用。此外，肽段对模式真菌灰霉病菌也有抗真菌作用。这是第一个证据表明王氏素对蜜蜂病原体具有抗生素作用。所描述的过程非常简单，不需要应用复杂的分离技术。它基于不同孔径的膜对RJ的透析，可以分离分子量在2 kDa以下、2 - 10 kDa之间和10 kDa以上的化合物。
A peptide fraction was isolated from honeybee royal jelly (RJ) using dual dialysis under acidic conditions. The N-terminal amino acid sequence of the major peptide within the fraction was V-T-C-D-L-L-S-F-K-G. This sequence corresponds to the honeybee defensin royalisin of MW 5523 Da which has been shown to exert antibacterial activity against some Gram-positive bacteria. Diffusion tests on agar plates showed that the peptide fraction had an inhibitory effect against the honeybee pathogen Paenibacillus larvae larvae, the primary pathogen of American foulbrood disease, as well as against other Gram-positive bacteria such as Bacillus subtilis and Sarcina lutea. Moreover, the peptide fraction was shown also to have antifungal effect against the model fungus Botrytis cinerea. It is the first evidence of an antibiotic effect of royalisin against a honeybee pathogen. The procedure described is very simple and does not require application of complicated separation techniques. It is based on dialysis of RJ using membranes with different pore sizes, which enable to separate the compounds having molecular weight below 2 kDa, between 2 kDa and 10 kDa, and over 10 kDa.