On the specificity of DNA-protein interactions.
On the specificity of DNA-protein interactions.
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DOI：
10.1073/pnas.83.6.1608
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发表时间：
1986-03
影响因子：
11.1
通讯作者：
P. V. Hippel;O. Berg
中科院分区：
文献类型：
--
作者：
P. V. Hippel;O. Berg
在本文中，我们总结了各种因素，必须考虑在建立的操作特异性的基因表达的蛋白质调节剂的DNA靶位点的结合。我们认为信息（组合）方面的结合位点的规范，实际的识别机制，和热力学的目标位点的选择对竞争的伪特异性和非（序列）特异性DNA结合位点的背景。结果提供了深入了解的设计，规格，并可能的演变的调节蛋白和它们的染色体结合的目标，以及到实际方面的设计的调节蛋白分离方案和物理化学的监管考虑在体内。
In this paper we summarize the various factors that must be considered in establishing the operational specificity of the binding of a protein regulator of gene expression to a DNA target site. We consider informational (combinatorial) aspects of binding-site specification, actual recognition mechanisms, and the thermodynamics of target-site selection against a background of competing pseudospecific and non-(sequence)-specific DNA binding sites. The results provide insight into the design, specification, and possibly the evolution of regulatory proteins and their chromosomal binding targets, as well as into practical aspects of the design of regulatory-protein isolation schemes and physicochemical regulatory considerations in vivo.