Crosslinked fibrin gels for tissue engineering: Two approaches to improve their properties
Crosslinked fibrin gels for tissue engineering: Two approaches to improve their properties
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DOI：
10.1002/jbm.a.35210
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发表时间：
2015-02-01
影响因子：
4.9
通讯作者：
Gallego Ferrer, Gloria
中科院分区：
文献类型：
--
作者：
Gamboa-Martinez, Tatiana C.;Luque-Guillen, Victoria;Gallego Ferrer, Gloria
纤维蛋白酶是一种蛋白质，可用作促进组织再生的理想支架材料。为了提高其物理性能，在这项研究中，天然交联剂，京尼平（GP），采用的目的是获得一个水凝胶与可调性能的组织工程应用。通过两种不同的方法使用四种浓度的GP交联纤维蛋白凝胶以得到稳定的水凝胶网络。测试了每种GP含量和所用方法的水凝胶的交联密度、机械性能、溶胀和酶降解。方法I：凝胶形成后的交联促进了高交联并长期保持凝胶形状，而方法II：同时凝胶形成和交联改善了凝胶的机械性能。该研究证实了使用不同浓度的GP作为纤维蛋白的合适交联剂，其促进L929体外培养21天的细胞活力。(C)2014 Wiley Periodicals，Inc.
Fibrin is a protein that can be used as an ideal scaffolding material to promote tissue regeneration. In order to enhance its physical properties in this study a natural crosslinker, genipin (GP), was employed with the aim to obtain a hydrogel with tuneable properties for tissue engineering applications. The fibrin gel was crosslinked by two different methods using four concentrations of GP to get a stable hydrogel network. Crosslinking density, mechanical properties, swelling, and enzymatic degradation of the hydrogels were tested for each GP content and method employed. The method I: Crosslinking after gel formation promotes a high crosslinking and retains the gel shape for long term whilst the method II: Simultaneous gel formation and crosslinking improves the mechanical properties of the gel. This study confirms the use of GP at different concentrations as a suitable crosslinker of fibrin that promotes the cellular viability of L929 for 21 days of in vitro culture. (C) 2014 Wiley Periodicals, Inc.