Optogenetic Examination of Prefrontal-Amygdala Synaptic Development
Optogenetic Examination of Prefrontal-Amygdala Synaptic Development
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DOI：
10.1523/jneurosci.3097-16.2017
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发表时间：
2017-03-11
影响因子：
5.3
通讯作者：
Clem, Roger L.
中科院分区：
文献类型：
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作者：
Arruda-Carvalho, Maithe;Wu, Wan-Chen;Clem, Roger L.
由内侧前额叶皮层（mPFC）和杏仁核组成的大脑网络在发育调节的认知和情绪过程中起着重要作用。然而，我们对mpfc -杏仁核回路的成熟知之甚少。我们对小鼠基底外侧杏仁核（BLA）新生儿至成年发育阶段的mPFC投影进行了解剖追踪，并对其与神经元的突触连接进行了光遗传学研究。结果表明，相对于其他mPFC通路，mPFC-BLA投射的出现延迟，并在婴儿期后期与BLA兴奋性和抑制性神经元建立突触传递，这些事件与整体突触驱动的大量增加相吻合。在随后的青春期，mPFC-BLA回路被兴奋性突触增强和前馈抑制的短暂激增进一步修改。后者与兴奋性神经元自发抑制电流的增加相关，表明mpfc - blaccircuit的成熟在gaba能传递旺盛的时期达到高潮。这些发现确定了mPFC-BLA传输的开始和完善的时间过程，并指出了这一关键网络发展的潜在敏感时期。
A brain network comprising the medial prefrontal cortex (mPFC) and amygdala plays important roles in developmentally regulated cognitive and emotional processes. However, very little is known about the maturation of mPFC-amygdala circuitry. We conducted anatomical tracing of mPFC projections and optogenetic interrogation of their synaptic connections with neurons in the basolateral amygdala (BLA) at neonatal to adult developmental stages in mice. Results indicate that mPFC-BLA projections exhibit delayed emergence relative to other mPFC pathways and establish synaptic transmission with BLA excitatory and inhibitory neurons in late infancy, events that coincide with a massive increase in overall synaptic drive. During subsequent adolescence, mPFC-BLA circuits are further modified by excitatory synaptic strengthening as well as a transient surge in feedforward inhibition. The latter was correlated with increased spontaneous inhibitory currents in excitatory neurons, suggesting that mPFC-BLAcircuit maturation culminates in a period of exuberant GABAergic transmission. These findings establish a time course for the onset and refinement of mPFC-BLA transmission and point to potential sensitive periods in the development of this critical network.