pH-dependent electron transfer reaction and direct bioelectrocatalysis of the quinohemoprotein pyranose dehydrogenase.
pH-dependent electron transfer reaction and direct bioelectrocatalysis of the quinohemoprotein pyranose dehydrogenase.
复制标题
pH 依赖性电子转移反应和醌血红素蛋白吡喃糖脱氢酶的直接生物电催化。
DOI：
10.1016/j.bbrc.2016.06.096
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发表时间：
2016
影响因子：
3.1
通讯作者：
N. Nakamura
中科院分区：
文献类型：
--
作者：
K. Takeda;H. Matsumura;T. Ishida;Makoto Yoshida;K. Igarashi;M. Samejima;H. Ohno;N. Nakamura
coprinopsis cinerea pyranose dehydrogenase （CcPDH）是一种细胞外醌血红蛋白，由ab型细胞色素结构域、pyroloquinoline -醌（PQQ）结构域和家族1型碳水化合物结合模块组成。采用不同的电子受体和碳电极，研究了ccpdh在不同pH水平下的电子转移反应。Phenazine methosulfate （PMS）直接在PQQ结构域反应，而cytochromec（cytc）则通过完整的ccpdh的细胞色素结构域反应。因此，电子从ccpdh催化区域的还原PQQ转移到n端细胞色素区域的血红素，后者作为内置介质将电子转移到异质电子转移蛋白。PMS还原的最佳pH值（pH 6.5）和细胞还原的最佳pH值（pH 8.5）不同。在pH为4.5 ~ 11的范围内，观察了催化氧化的电流。基于直接电子转移的ccpdh生物电催化表明，生物催化电流的pH谱与细胞性状的还原活性相似。
A pyranose dehydrogenase fromCoprinopsis cinerea(CcPDH) is an extracellular quinohemoeprotein, which consists ab-type cytochrome domain, a pyrroloquinoline-quinone (PQQ) domain, and a family 1-type carbohydrate-binding module. The electron transfer reaction ofCcPDH was studied using some electron acceptors and a carbon electrode at various pH levels. Phenazine methosulfate (PMS) reacted directly at the PQQ domain, whereas cytochromec(cytc) reacted via the cytochrome domain of intactCcPDH. Thus, electrons are transferred from reduced PQQ in the catalytic domain ofCcPDH to hemebin the N-terminal cytochrome domain, which acts as a built-in mediator and transfers electron to a heterogenous electron transfer protein. The optimal pH values of the PMS reduction (pH 6.5) and the cytcreduction (pH 8.5) differ. The catalytic currents for the oxidation ofl-fucose were observed within a range of pH 4.5 to 11. Bioelectrocatalysis ofCcPDH based on direct electron transfer demonstrated that the pH profile of the biocatalytic current was similar to the reduction activity of cytccharacters.
辅助活性家族 12：独特的 PQQ 依赖性氧化还原酶
DOI：
--
发表时间：
2017
期刊：
影响因子：
--
作者：
細井佳久;丸山毅;Makoto Yoshida
通讯作者：
Makoto Yoshida