Regulation by vitamin A of envelope cross-linking in cultured keratinocytes derived from different human epithelia
Regulation by vitamin A of envelope cross-linking in cultured keratinocytes derived from different human epithelia
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维生素 A 对源自不同人类上皮细胞的培养角质形成细胞中包膜交联的调节
DOI：
10.1128/mcb.2.9.1115-1117.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
F. Watt
中科院分区：
文献类型：
--
作者：
H. Green;F. Watt
当来自不同鳞状上皮的角质形成细胞在缺乏维生素A的情况下培养时，它们在终末分化的最后阶段形成交联的包膜。添加维生素会抑制包膜形成，但不同角质形成细胞亚型的抑制程度不同。在低浓度的乙酸视黄酯存在下，结膜角质形成细胞实际上不形成交联的包膜；食管和阴道角质形成细胞对维生素不太敏感，表皮角质形成细胞最不敏感。交联包膜形成的抑制与包膜前体——外皮蛋白浓度的成比例降低无关，而是发生在交联本身的水平上，这一过程依赖于细胞内钙离子浓度的增加。如果将 Ca2+ 引入细胞质，其中已被乙酸视黄酯阻止自发包膜交联的角质形成细胞会立即形成交联包膜。
When keratinocytes derived from different squamous epithelia are cultured in the absence of vitamin A, they form cross-linked envelopes during the last stage of terminal differentiation. Addition of the vitamin inhibits envelope formation, but the degree of inhibition is not the same for different keratinocyte subtypes. In the presence of low concentrations of retinyl acetate, conjunctival keratinocytes form virtually no cross-linked envelopes; esophageal and vaginal keratinocytes are less sensitive to the vitamin, and epidermal keratinocytes are the least sensitive. The suppression of cross-linked envelope formation is not associated with a proportional decrease in the concentration of involucrin, a precursor of the envelope, but occurs at the level of cross-linking itself, a process dependent on an increase in the intracellular concentration of calcium ions. Keratinocytes in which spontaneous envelope cross-linking has been prevented by retinyl acetate promptly form cross-linked envelopes if Ca2+ is introduced into the cytoplasm.