Isolating the whole complex of target proteins of FK506 using affinity resins from novel solid phases
Isolating the whole complex of target proteins of FK506 using affinity resins from novel solid phases
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DOI：
10.1007/s00216-006-0335-3
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发表时间：
2006-05-01
影响因子：
4.3
通讯作者：
Tanaka, A
中科院分区：
文献类型：
--
作者：
Takahashi, T;Shiyama, T;Tanaka, A
新型固相的发展使我们能够创建亲和树脂，可用于从大鼠脑裂解液中分离负责FK506免疫抑制作用的整个靶蛋白复合物，而从市售基质中获得的亲和树脂无法实现这种分离。结果表明，与由Affigel或Toyopearl制成的树脂相比，由这种新材料制成的亲和树脂在识别生物活性化合物的靶蛋白方面具有更高的有效性。这种有效性的产生是因为这种新材料具有足够的亲水性，可以减少非特异性结合蛋白，并且因为它具有更高密度的配体，可以捕获非普遍存在的靶蛋白。
The development of novel solid phases enabled us to create affinity resins that could be used to isolate the whole complex of target proteins responsible for the immunosuppressive effects of FK506 from rat brain lysate, whereas the affinity resins from commercially available matrices could not achieve this isolation. The results illustrate the enhanced effectiveness of the affinity resin made from this novel material at identifying the target protein of the bioactive compound compared to resins made from the well-known materials Affigel or Toyopearl. This effectiveness arises because the novel material is hydrophilic enough to reduce nonspecific binding proteins and because it has a higher density of ligands that capture the nonubiquitous target protein.