Complete Sequence of pEC012, a Multidrug-Resistant IncI1 ST71 Plasmid Carrying bla CTX-M-65, rmtB, fosA3, floR, and oqxAB in an Avian Escherichia coli ST117 Strain.
Complete Sequence of pEC012, a Multidrug-Resistant IncI1 ST71 Plasmid Carrying bla CTX-M-65, rmtB, fosA3, floR, and oqxAB in an Avian Escherichia coli ST117 Strain.
复制标题
pEC012 的完整序列，一种多重耐药 Inch ST71 质粒，在禽大肠杆菌 ST117 菌株中携带 bla(CTX-M-65)、rmtB、fosA3、floR 和 oqxAB
DOI：
10.3389/fmicb.2016.01117
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发表时间：
2016
影响因子：
5.2
通讯作者：
Hu GZ
中科院分区：
文献类型：
--
作者：
Pan YS;Zong ZY;Yuan L;Du XD;Huang H;Zhong XH;Hu GZ
对来自禽类大肠杆菌 D-ST117 菌株的 139,622 bp IncI1 ST71 接合质粒 pEC012 进行了测序，该质粒携带五个 IS26 括号内的抗性模块：IS26-fosA3-orf1-orf2-Δorf3-IS26， IS26-fip-ΔISEcp1-blaCTX-M-65-IS903D-iroN-IS26、IS26-ΔtnpR-blaTEM-1-rmtB-IS26、IS26-oqxAB-IS26 和 IS26-floR-aac(3)-IV-IS26。 pEC012 的主链与其他几个 IncI1 ST71 质粒：pV408、pM105 和 pC271 相似，但这些质粒具有不同的多药耐药区域排列。此外，还鉴定出新的 ISEc57 元件，它属于 IS21 家族。多重抗性区域的逐步出现证明了不同抗性决定因素通过同源重组的积累。据我们所知，这是第一项在禽类大肠杆菌 ST117 菌株中鉴定出携带 blaCTX-M-65、rmtB、fosA3、floR 和 oqxAB 的多重耐药 IncI1 ST71 质粒的研究。
A 139,622-bp IncI1 ST71 conjugative plasmid pEC012 from an avian Escherichia coli D-ST117 strain was sequenced, which carried five IS26-bracketed resistance modules: IS26-fosA3-orf1-orf2-Δorf3-IS26, IS26-fip-ΔISEcp1-blaCTX-M-65-IS903D-iroN-IS26, IS26-ΔtnpR-blaTEM-1-rmtB-IS26, IS26-oqxAB-IS26, and IS26-floR-aac(3)-IV-IS26. The backbone of pEC012 was similar to that of several other IncI1 ST71 plasmids: pV408, pM105, and pC271, but these plasmids had different arrangements of multidrug resistance region. In addition, the novel ISEc57 element was identified, which is in the IS21 family. The stepwise emergence of multi-resistance regions demonstrated the accumulation of different resistance determinants through homologous recombination. To the best of our knowledge, this is the first study to identify a multidrug-resistant IncI1 ST71 plasmid carrying blaCTX-M-65, rmtB, fosA3, floR, and oqxAB in an avian E. coli ST117 strain.