Physiological and molecular changes of detached wheat leaves in responding to various treatments.
Physiological and molecular changes of detached wheat leaves in responding to various treatments.
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DOI：
10.1111/j.1744-7909.2012.01139.x
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发表时间：
2012-08
影响因子：
11.4
通讯作者：
Lifeng Zhao;Haoshan Zhang;Bingjing Zhang;Xiaojuan Bai;Chunjiang Zhou
中科院分区：
文献类型：
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作者：
Lifeng Zhao;Haoshan Zhang;Bingjing Zhang;Xiaojuan Bai;Chunjiang Zhou
当叶片脱落时，叶片衰老被诱导或加速。然而，叶片离体衰老过程和衰老相关基因（SAGs）的表达模式尚不清楚。以小麦（Triticum aestivum L.）将叶片分离并用光、黑暗、低温（4 °C）、茉莉酸（JA）、脱落酸（阿坝）和水杨酸（SA）处理。分析了叶片表型、叶绿素含量、延迟荧光（DF）和两个SAG（即TaSAG3和TaSAG5）的表达水平。在不同处理下，离体叶片变黄的形态和叶绿素含量不同。黑暗、阿坝、JA和SA处理后，DF显著降低。TaSAG3和TaSAG5在自然衰老的叶片中表达，在不同的处理下表现出不同的表达模式。然而，TaSAG3和TaSAG5都在叶片脱落后上调。本研究揭示了不同胁迫条件下叶片衰老相关的生理变化和分子差异，从而导致叶片衰老。
Leaf senescence is induced or accelerated when leaves are detached. However, the senescence process and expression pattern of senescence-associated genes (SAGs) when leaves are detached are not clearly understood. To detect senescence-associated physiological changes and SAG expression, wheat (Triticum aestivum L.) leaves were detached and treated with light, darkness, low temperature (4 °C), jasmonic acid (JA), abscisic acid (ABA), and salicylic acid (SA). The leaf phenotypes, chlorophyll content, delayed fluorescence (DF), and expression levels of two SAGs, namely, TaSAG3 and TaSAG5, were analyzed. Under these different treatments, the detached leaves turned yellow with different patterns and varying chlorophyll content. DF significantly decreased after the dark, ABA, JA and SA treatments. TaSAG3 and TaSAG5, which are expressed in natural senescent leaves, showed different expression patterns under various treatments. However, both TaSAG3 and TaSAG5 were upregulated after leaf detachment. Our results revealed senescence-associated physiological changes and molecular differences in leaves, which induced leaf senescence during different stress treatments.