Orchestrated transcription of key pathways in Arabidopsis by the circadian clock
Orchestrated transcription of key pathways in Arabidopsis by the circadian clock
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DOI：
10.1126/science.290.5499.2110
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发表时间：
2000-12-15
期刊：
影响因子：
56.9
通讯作者：
Kay, SA
中科院分区：
文献类型：
--
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
像大多数有机体一样，植物有协调内部事件和外部环境的内源生物钟。我们使用高密度寡核苷酸微阵列来检测拟南芥的基因表达，发现在阵列上的8000多个基因中，有6%的基因在稳态信使RNA水平上显示出昼夜节律变化。在植物对光的反应和其他关键的代谢途径中，发现了一系列昼夜节律调节的基因。对循环基因的计算分析使我们能够识别出高度保守的启动子基序，我们发现这是基因表达的昼夜调控所必需的。我们的研究全面介绍了真核生物生物钟对生理通路的时间区划。
Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA Levers. Clusters of circadian-regulated genes were found in pathways involved in plant responses to Light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.