Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons
Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons
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DOI：
10.1073/pnas.94.4.1527
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发表时间：
1997-02-18
影响因子：
11.1
通讯作者：
Mandel, G
中科院分区：
文献类型：
--
作者：
ToledoAral, JJ;Moss, BL;Mandel, G
不同组织中的膜兴奋性在很大程度上是由于编码电压依赖性钠通道的不同基因的选择性表达。尽管已经确定了大脑、骨骼肌和心肌中的主要钠通道，但负责周围神经系统内兴奋性的主要钠通道类型仍然难以捉摸。我们现在描述了钠通道、周围神经1型（PN1）的推导的一级结构，它在整个周围神经系统中高水平表达，并针对培养的背根神经节神经元的神经末梢，使用培养的 PC12 细胞的研究表明，用神经生长因子处理细胞可以诱导 PN1 的表达和靶向，优先定位表明 PN1 钠通道在神经兴奋性中发挥特定作用。
Membrane excitability in different tissues is due, in Large part, to the selective expression of distinct genes encoding the voltage-dependent sodium channel, Although the predominant sodium channels in brain, skeletal muscle, and cardiac muscle have been identified, the major sodium channel types responsible for excitability within the peripheral nervous system have remained elusive, We now describe the deduced primary structure of a sodium channel, peripheral nerve type 1 (PN1), which is expressed at high levels throughout the peripheral nervous system and is targeted to nerve terminals of cultured dorsal root ganglion neurons, Studies using cultured PC12 cells indicate that both expression and targeting of PN1 is induced by treatment of the cells with nerve growth factor, The preferential Localization suggests that the PN1 sodium channel plays a specific role in nerve excitability.