Diabetes increases collagenase activity in extracts of rat gingiva and skin.
Diabetes increases collagenase activity in extracts of rat gingiva and skin.
复制标题
糖尿病会增加大鼠牙龈和皮肤提取物中胶原酶的活性。
DOI：
10.1111/j.1600-0765.1983.tb00331.x
复制
发表时间：
1983
影响因子：
3.5
通讯作者：
Golub,LM
中科院分区：
文献类型：
--
作者：
Ramamurthy,NS;Golub,LM
The connective tissues of diabetic humans and experimental animals exhibit abnormalities in collagen metabolism including an increased production of collagenolytic enzymes by gingival explants in tissue culture. In the current study, gingival tissue and skin were obtained from control and diabetic rats, the latter on the 5th, 10th, 15th, and 22nd day after inducing the disease with streptozotocin. Extracts of the tissues were assessed for collagenase activity using either14C‐labeled collagen fibrils or Peptide‐P as substrate and by detecting collagenase digestion products by SDS‐polyacrylamide gel electrophoresis. Elastase was measured using the specific substrate, succinyl‐(L‐Alanyl)3‐p‐nitroanilide. Diabetes increased the activity of collagenase in extracts of gingiva and skin; elastase, however, was increased dramatically only in the latter tissue. These findings (1) indicate that diabetes‐induced enhanced gingival collagenase activity occursin vivoas well asin vitroand (2) explain, at least in part, the greater loss of collagen in skin than in gingiva of the diabetic rat and other complications such as unusually severe periodontal breakdown.
