Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells
Comparison of Intracardiac Cell Transplantation: Autologous Skeletal Myoblasts Versus Bone Marrow Cells
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DOI：
10.1161/01.cir.0000087657.29184.9b
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发表时间：
2003-09
期刊：
影响因子：
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通讯作者：
Richard B. Thompson;S. Emani;B. Davis;E. J. van den Bos;Y. Morimoto;D. Craig;D. Glower;Doris A Taylor
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文献类型：
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作者：
Richard B. Thompson;S. Emani;B. Davis;E. J. van den Bos;Y. Morimoto;D. Craig;D. Glower;Doris A Taylor
背景：多种细胞类型被提出用于心脏修复，但缺乏并排比较。我们验证了自体骨髓或骨骼肌来源的祖细胞在心脏内移植对局部心脏功能的改善程度相似的假设。方法与结果：39只新西兰大白兔左心室冷冻损伤，同时后肢骨髓穿刺或比目鱼肌活检。肌细胞和骨髓细胞均在体外扩增。2周后，将108个骨骼肌（SM组）或骨髓源性祖细胞（BM组）注射到冻伤区（SM: n=12; BM: n=8）。其余动物单独注射培养基（对照组：n=16）。在基线、细胞注射前和注射后4周，采用微压法和声压法测量局部收缩功能。在SM组和BM组中，细胞治疗导致类似程度的卒中工作的改善（分别与对照组相比P =0.0026和P =0.0085）。BM组与SM组间差异无统计学意义（P =0.9）。组织学上，所有细胞处理动物均有移植细胞。注射后的成肌细胞在瘢痕各处形成肌管或肌细胞，表达慢速和快速肌球蛋白重链。骨髓细胞的一个亚群向肌源性表型分化，正如在移植区域的desmin和-肌动蛋白的表达所表明的那样。结论：骨髓源性祖细胞移植和成肌分化对心肌损伤后局部心脏收缩功能的改善程度与骨骼肌细胞相似。
Background—Multiple cell types are being proposed for cardiac repair, but side-by-side comparisons are lacking. We tested the hypothesis that intracardiac transplantation of autologous bone marrow- or skeletal muscle-derived progenitor cells improve regional heart function to a similar degree. Methods and Results—Thirty-nine New Zealand White rabbits underwent cryoinjury of the left ventricle and simultaneous hind limb bone marrow aspiration or soleus muscle biopsy. Both muscle and bone marrow cells were expanded in vitro. After 2 weeks, 108 skeletal muscle (SM group) or bone marrow-derived progenitor cells (BM group) were injected into the cryoinjured region (SM: n=12; BM: n=8). Medium alone was injected into the remaining animals (Control: n=16). Regional systolic function was measured using micromanometry and sonomicrometry at baseline, before, and 4 weeks after cell injection. Cell treatment resulted in a similar degree of improvement in a derivative of stroke work in the SM and BM groups (P =0.0026 and P =0.0085 versus Control, respectively). No significant difference was seen between BM and SM groups (P =0.9). On histology, engrafted cells were found in all of the cell treated animals. Injected myoblasts formed myotubes or muscle cells throughout the scar that expressed slow and fast myosin heavy chain. A subset of bone marrow cells differentiated toward a myogenic phenotype, as indicated by expression of desmin and -sarcomeric actin in the engrafted areas. Conclusion—Transplantation and myogenic differentiation of bone marrow-derived progenitor cells increased regional systolic heart function after myocardial injury to a similar degree as skeletal myoblasts.