Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
Characterization the carotenoid productions and profiles of three Rhodosporidium toruloides mutants from Agrobacterium tumefaciens-mediated transformation
复制标题
根癌农杆菌介导转化的三种圆红冬孢酵母突变体的类胡萝卜素产量和概况的表征
DOI：
10.1002/yea.3236
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发表时间：
2017-08-01
期刊：
影响因子：
2.6
通讯作者：
Zhu, Beiwei
中科院分区：
文献类型：
--
作者：
Lin, Xinping;Gao, Ning;Zhu, Beiwei
红酵母Rhodosporidium toruloides是一种已知的脂质生产者，能够积累大量的三酰基甘油和类胡萝卜素。然而，由于有限的生化信息和低效的遗传工具，研究其类胡萝卜素的生产概况仍然具有挑战性。在这里，我们使用农杆菌介导的转化（ATMT），以改变其类胡萝卜素的生产和配置文件。我们构造了R。利用ATMT技术对圆红酵母NP 11突变体文库进行了筛选，筛选出3株不同颜色的突变体，并通过高压液相色谱-质谱（HPLC-MS）联用技术对它们的类胡萝卜素产物进行了分析，确定了这些菌株在类胡萝卜素产量及其组成方面的差异。然后，我们使用基因组步行技术定位T-DNA插入位点，并提供了新的表型方面的讨论。本研究首次改变了红球藻中类胡萝卜素的产生模式。圆酵母。版权所有（C）2017约翰威利父子有限公司
The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciensmediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected three mutants with different colours, characterized their carotenoid products by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis and assured differences among those strains in terms of carotenoid production and its composition profiles. We then located T-DNA insertion sites using the genome walking technology and provided discussions in terms of the new phenotypes. This study is the first of its kind to change the carotenoid production profiles in R. toruloides. Copyright (C) 2017 John Wiley & Sons, Ltd.