Desensitization of the P2X(2) receptor controlled by alternative splicing
Desensitization of the P2X(2) receptor controlled by alternative splicing
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DOI：
10.1016/s0014-5793(97)00128-2
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发表时间：
1997-03-10
期刊：
影响因子：
3.5
通讯作者：
Glowatzki, E
中科院分区：
文献类型：
--
作者：
Brandle, U;Spielmanns, P;Glowatzki, E
P2X受体是由细胞外ATP门控的离子通道。我们在此报道克隆了细胞内c端携带207 bp缺失的P2X(2)受体剪接变体（P2X(2-2)），并分析了P2X(2)基因的相应基因组结构。P2X（2-2）与原P2X(2)序列一样在多种组织中高表达。外源表达的P2X(2)或P2X（2-2）受体介导的ATP激活电流在ATP持续存在下的脱敏时间常数和稳态电流存在显著差异。这些结果暗示不同表达这些P2X(2)亚型的细胞之间存在功能差异。(C) 1997年欧洲生化学会联合会。
P2X receptors are ion channels gated by extracellular ATP. We report here cloning of a P2X(2) receptor splice variant (P2X(2-2)) carrying a 207 bp deletion in the intracellular C-terminus and the analysis of the corresponding genomic structure of the P2X(2) gene. P2X(2-2) is as highly expressed as the original P2X(2) sequence in various tissues. ATP-activated currents mediated by heterologous expressed P2X(2) or P2X(2-2) receptors showed significant differences in desensitization time constants and steady-state currents in the continuous presence of ATP. These results imply functional differences between cells differentially expressing these P2X(2) isoforms. (C) 1997 Federation of European Biochemical Societies.