Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis
Bacterial diversity of wild-caught Lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in Brazil) under distinct physiological conditions by metagenomics analysis
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DOI：
10.1186/s13071-017-2593-7
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发表时间：
2017-12-29
影响因子：
3.2
通讯作者：
Costa Secundino, Nagila Francinete
中科院分区：
文献类型：
--
作者：
Araujo Machado Pires, Ana Clara;Martinez Villegas, Luis Eduardo;Costa Secundino, Nagila Francinete
背景资料：利什曼病是由利什曼原虫属的原生动物引起的一组疾病，其通过白蛉白蛉的叮咬传播。在新大陆，长须Lutzomyia longipalpis是内脏利什曼病最重要的媒介，并且是巴西已证实的婴儿查加斯利什曼原虫的媒介。在载体内发育期间，利什曼原虫可以与各种微生物如真菌和细菌相互作用。细菌的存在下，在中肠的白蛉可以影响寄生虫的发展和生存。结果：细菌为靶向的宏基因组分析揭示了不同的社会组成之间的不同的生理阶段的测试。以扩增子为导向的宏基因组分析显示，64个细菌属和46个科。通过交叉从每个实验条件下的分类群指数的核心组成的6个属（肠杆菌属，沙雷氏菌属，寡养单胞菌属，Enhydrobacter，假单胞菌属和金黄杆菌属）进行了鉴定。结论：所观察到的动态性质的细菌群落扩大了知识有关的三方主机-微生物-病原体的相互作用。进一步研究解决如何实验室和现场收集的社区不同的是至关重要的成功开发控制策略的基础上细菌共生体和parattransgenesis，已经在其他节肢动物载体测试。
Background: The leishmaniases are a group of diseases caused by protozoans of the genus Leishmania, which are transmitted by the bite of phlebotomine sand flies. In the New World, Lutzomyia longipalpis is the most important vector of visceral leishmaniasis and is a proven vector for Leishmania infantum chagasi in Brazil. During development within the vector, Leishmania can interact with a variety of microorganisms such as fungi and bacteria. The presence of bacteria in the midgut of sand flies can influence the development and survival of the parasite.Results: The bacteria-targeted metagenomic analysis revealed different community compositions between the distinct physiological stages of those tested. The amplicon-oriented metagenomic profiling revealed 64 bacterial genera and 46 families. By crossing the taxa indices from each experimental condition a core composed of 6 genera was identified (Enterobacter, Serratia, Stenotrophomonas, Enhydrobacter, Pseudomonas and Chryseobacterium).Conclusions: The observed dynamic nature of the bacterial community expands the knowledge pertaining to the tripartite host-microbiota-pathogen interactions. Further studies addressing how laboratory and field collected communities differ are critical to successfully develop control strategies based on bacterial symbionts and paratransgenesis, as already tested in other arthropod vectors.