Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)
Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)
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DOI：
10.1073/pnas.93.25.14862
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发表时间：
1996-12-10
影响因子：
11.1
通讯作者：
Moore, PS
中科院分区：
文献类型：
--
作者：
Russo, JJ;Bohenzky, RA;Moore, PS
用BC-1细胞株的粘粒和噬菌体基因组文库定位了Kaposi肉瘤相关疱疹病毒(KSHV或HHV-8)的基因组，测得其核苷酸序列，但基因组右端的3kb区域难以克隆，BC-1KSHV基因组由一个140.5 kb的唯一编码区组成，两侧有多个富含G+C的801BP末端重复序列，该细胞培养衍生株存在明显的基因组重复。该病毒编码补体结合蛋白、三种细胞因子(两种巨噬细胞炎性蛋白和白介素6)、二氢叶酸还原酶、bcl2、干扰素调节因子、白介素8受体、神经细胞黏附分子样黏附素、D型细胞周期蛋白以及病毒结构和代谢蛋白的同源物，以及病毒结构和代谢蛋白。
The genome of the Kaposi sarcoma-associated herpesvirus (KSHV or HHV8) was mapped with cosmid and phage genomic libraries from the BC-1 cell line, Its nucleotide sequence was determined except for a 3-kb region at the right end of the genome that was refractory to cloning, The BC-1 KSHV genome consists of a 140.5-kb-long unique coding region flanked by multiple G+C-rich 801-bp terminal repeat sequences, A genomic duplication that apparently arose in the parental tumor is present in this cell culture-derived strain. At least 81 ORFs, including 66 with homology to herpesvirus saimiri ORFs, and 5 internal repent regions are present in the long unique region, The virus encodes homologs to complement-binding proteins, threecytokines (two macrophage inflammatory proteins and interleukin 6), dihydrofolate reductase, bcl-2, interferon regulatory factors, interleukin 8 receptor, neural cell adhesion moleculelike adhesin, and a D-type cyclin, as well as viral structural and metabolic proteins, Terminal repeat analysis of virus DNA from a KS lesion suggests a monoclonal expansion of KSHV in the KS tumor.