Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus
Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus
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DOI：
10.1093/emboj/18.19.5370
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发表时间：
1999-10-01
期刊：
影响因子：
11.4
通讯作者：
Sommer, H
中科院分区：
文献类型：
--
作者：
Egea-Cortines, M;Saedler, H;Sommer, H
在金鱼草中，花分生组织是由分生组织识别基因建立的。花分生组织在轮生中产生花器官，其身份是通过器官识别基因的组合活动确定的。花分生组织识别基因 SQUAMOSA 和器官识别基因 DEFICIENS 或 GLOBOSA 的双突变体产生的花中轮生图案部分丢失。在酵母中，SQUA、DEF 和 GLO 蛋白通过其 C 末端形成三元复合物，在凝胶迁移测定中显示，与 DEF/GLO 异二聚体或 SQUA/SQUA 同二聚体相比，DNA 与 CArG 基序的结合增加。植物MADS-box因子形成的三元复合物增加了其调节功能的复杂性，并且可能是建立轮生叶序和花器官特征基因组合相互作用的分子基础。
In Antirrhinum, floral meristems are established by meristem identity genes. Floral meristems give rise to floral organs in whorls, with their identity established by combinatorial activities of organ identity genes. Double mutants of the floral meristem identity gene SQUAMOSA and organ identity genes DEFICIENS or GLOBOSA produce flowers in which whorled patterning is partially lost. In yeast, SQUA, DEF and GLO proteins form ternary complexes via their C-termini, which in gel-shift assays show increased DNA binding to CArG motifs compared with DEF/GLO heterodimers or SQUA/SQUA homodimers. Formation of ternary complexes by plant MADS-box factors increases the complexity of their regulatory functions and might be the molecular basis for establishment of whorled phyllotaxis and combinatorial interactions of floral organ identity genes.