The Protective Effect of Brazilian Propolis against Glycation Stress in Mouse Skeletal Muscle
The Protective Effect of Brazilian Propolis against Glycation Stress in Mouse Skeletal Muscle
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DOI：
10.3390/foods8100439
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发表时间：
2019-10-01
期刊：
影响因子：
5.2
通讯作者：
Hayashi, Tatsuya
中科院分区：
文献类型：
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作者：
Egawa, Tatsuro;Ohno, Yoshitaka;Hayashi, Tatsuya
我们研究了巴西蜂胶（一种由蜜蜂产生的天然蜂胶物质）对小鼠骨骼肌糖基化应激的保护作用。将小鼠分为四组：（1）正常饮食+饮用水，（2）含巴西棕榈油（0.1%）的饮食+饮用水，（3）正常饮食+含甲基乙二醛（MGO）（0.1%）的饮用水，和（4）含巴西棕榈油（0.1%）的饮食+含MGO（0.1%）的饮用水。MGO治疗20周减少了趾长伸肌（EDL）的重量，并倾向于在比目鱼肌。摄入巴西棕榈油对EDL肌肉的这种变化没有影响，但倾向于增加比目鱼肌的重量，无论MGO治疗。在EDL肌肉中，巴西人的摄入抑制了MGO治疗小鼠中MGO衍生的晚期糖基化终产物（AGEs）的积累。MGO不影响EDL肌肉中乙二醛酶1的活性，但巴西蜂胶可增强EDL肌肉中乙二醛酶1的活性。MGO治疗增加了炎症相关分子、白细胞介素（IL）-1 β、IL-6和Toll样受体4（TLR 4）的mRNA表达。巴西人的摄入抑制了这些增加。MGO和/或ESTA对AGEs的积累，glycoproteinase 1活性和比目鱼肌的炎症反应没有影响。这些结果表明，巴西芦荟通过抑制AGEs的积累，促进MGO解毒，减少骨骼肌中的促炎反应，对糖化应激发挥保护作用。然而，这些抗糖化作用并不能阻止糖化诱导的肌肉质量减少。
We investigated the protective effect of Brazilian propolis, a natural resinous substance produced by honeybees, against glycation stress in mouse skeletal muscles. Mice were divided into four groups: (1) Normal diet + drinking water, (2) Brazilian propolis (0.1%)-containing diet + drinking water, (3) normal diet + methylglyoxal (MGO) (0.1%)-containing drinking water, and (4) Brazilian propolis (0.1%)-containing diet + MGO (0.1%)-containing drinking water. MGO treatment for 20 weeks reduced the weight of the extensor digitorum longus (EDL) muscle and tended to be in the soleus muscle. Ingestion of Brazilian propolis showed no effect on this change in EDL muscles but tended to increase the weight of the soleus muscles regardless of MGO treatment. In EDL muscles, Brazilian propolis ingestion suppressed the accumulation of MGO-derived advanced glycation end products (AGEs) in MGO-treated mice. The activity of glyoxalase 1 was not affected by MGO, but was enhanced by Brazilian propolis in EDL muscles. MGO treatment increased mRNA expression of inflammation-related molecules, interleukin (IL)-1 beta, IL-6, and toll-like receptor 4 (TLR4). Brazilian propolis ingestion suppressed these increases. MGO and/or propolis exerted no effect on the accumulation of AGEs, glyoxalase 1 activity, and inflammatory responses in soleus muscles. These results suggest that Brazilian propolis exerts a protective effect against glycation stress by inhibiting the accumulation of AGEs, promoting MGO detoxification, and reducing proinflammatory responses in the skeletal muscle. However, these anti-glycation effects does not lead to prevent glycation-induced muscle mass reduction.